The response of rhesus monkey sperm motility to cervical mucus and solid surfaces

Abstract

Movement characteristics of rhesus monkey spermatozoa were analyzed using high-speed cinemicrography. In the first experiment, spermatozoa were studied at 100 frames/sec in diluted semen near a surface, and after entering ovulatory cervical mucus from a bonnet monkey. In mucus, the spermatozoa swam more slowly, with reduced flagellar beat frequencies. The beat shape was altered, and there was less lateral yawing of the sperm head. In the second experiment, spermatozoa in diluted semen were studied at 500 frames/sec in deep preparations, while swimming near a surface or when in the midplane of these preparations. Those sperm in the midplane swam faster, but with lower beat frequencies than those near the surface, and exhibited much more pronounced yawing motions. Such distinctions in sperm motion are probably hydromechanical in origin and may be significant during transport in the female.