Determination of Self-Compatibility of the 'Arsel' Olive Cultivar Obtained by Hybridization Breeding

Hükümran Gül, M. İsfendiyaroğlu, N. Acarsoy Bilgin, Rüştü Efe Değer
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Abstract

This study was aimed to determine the fertilization biology of the 'Arsel' olive cultivar obtained by hybridization breeding. For this reason, treatments of open pollination, cross pollination, and self-pollination were performed for 2 years and self-compatibility and appropriate pollinators of this new cultivar were investigated. Among the varieties included as pollinators (‘Memecik’, ‘Gemlik’, ‘Uslu’, ‘Ayvalık’ and ‘Eğriburun Nizip’), the highest fruit set was achieved with ‘Memecik’ and ‘Gemlik’ varietes in both years (2.64%, 1.37% and 1.75%, 1.39%). For this reason, ‘Memecik’ and ‘Gemlik’ cultivars were the best pollinator for ‘Arsel’ olive was thought. On the other hand, considering the statistical analyzes and productivity index values, ‘Arsel’ variety was found to be self-incompatible. However, the data also indicated that cross pollination was effective in increasing fruit set. Therefore, it is thought that the use of pollinator cultivar in orchard establishment would be beneficial in terms of yield. Pollen viability and germination tests were performed by using 2,3,5 Triphenyl Tetrazolium Chloride (TTC) and agar in petri (15% sucrose + 1% agar + 100 ppm H3BO3) methods in the study. The highest pollen viability rate was observed in ‘Memecik’ cultivar in 2020 and there isn’t statistical differences between olive cultivars in 2018. The highest pollen germination rate was observed in ‘Arsel’ cultivar in 2018. Accordingly, differences were determined between pollen viability and germination rates of examined olive cultivars in terms of years.
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杂交育种获得的‘Arsel’橄榄品种自交亲和性的测定
本研究旨在确定杂交育种获得的‘Arsel’橄榄品种的受精生物学特性。为此,对该新品种进行了2年的开放授粉、异花授粉和自花授粉处理,并对其自交亲和性和适宜传粉者进行了研究。在作为传粉媒介的品种(' Memecik '、' Gemlik '、' Uslu '、' Ayvalık '和' Eğriburun Nizip ')中,' Memecik '和' Gemlik '品种在两年中均取得了最高的坐果率(2.64%,1.37%和1.75%,1.39%)。因此,' Memecik '和' Gemlik '品种被认为是' Arsel '橄榄的最佳传粉者。另一方面,考虑到统计分析和生产力指数值,发现' Arsel '品种是自交不亲和的。然而,数据也表明异花授粉在增加坐果方面是有效的。因此,我们认为在果园建设中使用传粉品种将有利于产量的提高。采用2、3、5三苯基氯化四氮唑(TTC)和琼脂(15%蔗糖+ 1%琼脂+ 100 ppm H3BO3)培养皿法进行花粉活力和萌发试验。‘Memecik’品种花粉活力率在2020年最高,2018年品种间差异无统计学意义。2018年,‘Arsel’品种花粉发芽率最高。因此,确定了不同橄榄树品种花粉活力和发芽率的差异。
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