Researches Concerning Improving Methods of In Vitro Microtubers Production

Abstract

Five genotypes of potato (Solanum tuberosum L.) were induced to form microtubers under two in vitro culture conditions (continuos darkness and light). Cultures maintained under continuos darkness (with a temperatue of 170C) had a higher yield with a greater number of microtuber (1.394/plantlet) than those maintained under long days (16 h of light/day) combined with temperature of 200C. In the last case the microtubers were higher, with with the highest average weight of 0.602 g. Three phytohormones it was used: coumarine, benzylaminopurine and salicylic acid. Regarding the average number of microtubers obtained when it was used coumarine and salicylic acid, the highest average number of microtubers was recorded (1,135 microtubers/plantlet). The study had been performed also on two fractions of the of sugar quantity (80 and 140 g/l), but they had less influence compared to the varieties.