DETERMINATION OF GENETIC DIFFERENCES BETWEEN Artemisia monosperma PLANTS BY USING GENETIC FINGEPRINTING.

Abstract

In the present study, three of the Egyptian Artemisia monosperma genotypes were selected from Bir al-Abd city in North Sinai, Egypt, for genetics analysis by using 5 of inter simple sequence repeat (ISSR) markers. The obtained results indicated that the ISSR primers were able to amplify DNA fragments showing, polymorphic DNA amplification patterns among the genotypes. The data indicated that out of the total 120 bands, 64 were polymorphic (64%). The number of the observed bands ranged from two for the primer UBC849 to one in the primer UBC811with an average of 2.4 across for the tested genotypes. The data also showed the presence of reasonable number of alleles for every locus in the present study, which may be due of the nature to amplified ISSR loci that most to them are located to the expressed sequence tags (EST) in the transcribed regions of the genomic DNA sequences. UBC849 with average value to 7. Consequently, most of the five ISSR loci in this study were useful for the evaluation of genetic diversity between the three Egyptian Artemisia monosperma genotypes. However, it is worth mentioning, this value expresses the existence to different alleles of one or more loci of homologous chromosomes. Polymorphism data content (PIC) value of the Artemisia monosperma genomic ISSR indicated temperate to high level of in formativeness with average PIC value to 0.42 over all the tested ISSR loci primers, where it ranged from 0.07 by using to UBC849 to 0.42 in UBC811 for all genotypes under research by a mean to 0.18. Also, this study signalized a low heterozygosis level of the genotypes under research, so that for the variation between the number of alleles in each locus and number of effective alleles, so that to the existences to exclusive/specific alleles to the genotypes. The genetic analysis of the three Artemisia momosperma based on 5 ISSR markers detected 64 separate specific alleles, and also, that the three genotypes (G.1, G.2 and G.3) had alleles were unrivaled. So these five genotypes did not give conformable DNAfingerprints. It is noted that, largest number patented to the specific/unique alleles were of the genotype number 3, where 30 specific alleles were patented to this genotype only.