Methylomonas rapida sp. nov., a novel species of fast-growing, carotenoid-producing obligate methanotrophs with high biotechnological potential

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2023-04-01 DOI:10.1016/j.syapm.2023.126398
Ekaterina N. Tikhonova , Ruslan Z. Suleimanov , Kirill K. Miroshnikov , Igor Y. Oshkin , Svetlana E. Belova , Olga V. Danilova , Aleksandr A. Ashikhmin , Aleksey A. Konopkin , Sergey Y. But , Valentina N. Khmelenina , Nikolai V. Pimenov , Svetlana N. Dedysh
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引用次数: 5

Abstract

The genus Methylomonas accommodates strictly aerobic, obligate methanotrophs, with their sole carbon and energy sources restricted to methane and methanol. These bacteria inhabit oxic-anoxic interfaces of various freshwater habitats and have attracted considerable attention as potential producers of a single-cell protein. Here, we characterize two fast-growing representatives of this genus, strains 12 and MP1T, which are phylogenetically distinct from the currently described Methylomonas species (94.0–97.3 % 16S rRNA gene sequence similarity). Strains 12 and MP1T were isolated from freshwater sediments collected in Moscow and Krasnodar regions, respectively. Cells of these strains are Gram-negative, red-pigmented, highly motile thick rods that contain a type I intracytoplasmic membrane system and possess a particulate methane monooxygenase (pMMO) enzyme. These bacteria grow between 8 and 45 °C (optimum 35 °C) in a relatively narrow pH range of 5.5–7.3 (optimum pH 6.6–7.2). Major carotenoids synthesized by these methanotrophs are 4,4′-diaplycopene-4,4′-dioic acid, 1,1′-dihydroxy-3,4-didehydrolycopene and 4,4′-diaplycopenoic acid. High biomass yield, of up to 3.26 g CDW/l, is obtained during continuous cultivation of MP1T on natural gas in a bioreactor at a dilution rate of 0.22 h−1. The complete genome sequence of strain MP1T is 4.59 Mb in size; the DNA G + C content is 52.8 mol%. The genome encodes four rRNA operons, one pMMO operon and 4,216 proteins. The genome sequence displays 82–85 % average nucleotide identity to those of earlier described Methylomonas species. We propose to classify these bacteria as representing a novel species of the genus Methylomonas, M. rapida sp. nov., with the type strain MP1T (=KCTC 92586T = VKM B-3663T).

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快速甲基单胞菌是一种快速生长、产类胡萝卜素的专性甲烷氧化菌,具有很高的生物技术潜力
甲基单胞菌属适应严格的需氧、专性甲烷营养菌,其唯一的碳和能源仅限于甲烷和甲醇。这些细菌栖息在各种淡水栖息地的缺氧-缺氧界面,作为单细胞蛋白质的潜在生产者,引起了人们的极大关注。在这里,我们描述了该属的两个快速生长的代表,菌株12和MP1T,它们在系统发育上与目前描述的甲基单胞菌物种不同(94.0-97.3%的16S rRNA基因序列相似性)。菌株12和MP1T分别从莫斯科和克拉斯诺达尔地区采集的淡水沉积物中分离出来。这些菌株的细胞是革兰氏阴性、红色、高度活动的粗棒,含有I型细胞质内膜系统,并具有颗粒甲烷单加氧酶(pMMO)。这些细菌生长在8至45°C(最适35°C)之间,pH范围相对较窄,为5.5–7.3(最适pH 6.6–7.2。在生物反应器中以0.22 h−1的稀释率在天然气上连续培养MP1T,可获得高达3.26 g CDW/l的生物质产量。菌株MP1T的全基因组序列大小为4.59Mb;DNA G+C含量为52.8mol%。该基因组编码4个rRNA操纵子、1个pMMO操纵子和4216个蛋白质。该基因组序列显示出82–85%的平均核苷酸同一性,与先前描述的甲基单胞菌物种相同。我们建议将这些细菌分类为代表甲基单胞菌属的一个新物种,M.rapida sp.nov.,其类型菌株为MP1T(=KCTC 92586T=VKM B-3663T)。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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