Daphne Verdoorn, Cindy Gj Cleypool, Claire Mackaaij, Ronald Law Bleys
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引用次数: 0
Abstract
The anatomy of the carotid body (CB) and its nerve supply are important, because it is a potential therapeutic target for treatment of various clinical conditions. Visualization of the CB in situ in fixed human anatomical specimens is hampered by obscuring adipose and connective tissues. We developed a tissue clearing method to optimize identification of the CB. We used single sided carotid bifurcations of six human cadavers fixed long term. Visualization of the CB was accomplished by clearing tissue with xylene. Under incident light, carotid bifurcations exhibited a less transparent, darker colored CB; hematoxylin and eosin stained paraffin sections confirmed its identity. Our visualization of the CB in situ in human carotid bifurcations fixed long term enabled targeted resection and subsequent topographic and morphometric measurements of the CB. Our procedure does not interfere with immunohistochemical staining of sections prepared from such specimens.
期刊介绍：
Biotechnic & Histochemistry (formerly Stain technology) is the
official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926.
Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well.
Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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