Trophoblast Cell Lines JAR and JEG-3 Modulate CD90 (Thy‑1) Expression on Decidual Stromal Cells

Abstract

CD90 is a glycoprotein involved in leukocyte homing and cell differentiation. In human endometrium CD90 is described on endothelial and stromal cells. If pregnancy occurs, the tissue is infiltrated by immune and trophoblast cells, which cooperate to remodel the tissue. The process requires a fine regulation of cell adhesion and migration. The expression of CD90 on cultured decidual stromal cells and its modulation by trophoblast cells from JAR and JEG-3 cell lines via soluble factors are investigated by flow cytometry. Some of decidual stromal cell lines showed ubiquitous expression of CD90 but in others a coexistence of CD90(high) and CD90(low) decidual stromal cells was observed. Further, a reduction of CD90 expression on decidual stromal cells was defined when decidual stromal cells communicate with trophoblast cells via soluble factors. The results sugguest a trophoblast guided differentiation of decidual stromal cells that could influence immune cells migration and tissue distribution.