In vitro induction and intraocular application in oxygen-induced retinopathy of adipose-derived mesenchymal stem cells.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Vision Pub Date : 2022-01-01

Lvlv Zhou, Haifeng Zhang, Sarina Wu, Yuhong He, Kai Guo

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Abstract

Purpose: We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application.

Methods: HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. HADSCs were intraocularly injected into oxygen-induced retinopathy (OIR) mice to check the movement, fusion, proliferation, and prognosis in vivo. Electron microscopy was used to check retinal changes to confirm the safety of hADSCs in intraocular application.

Results: After induction, hADSCs expressed von Willebrand Factor (vWF), the cell marker of ECs. The hADSCs were distributed above the retina after an intravitreal injection in the OIR mice. The injected cells did not fuse with the retina and gathered in the central and peripheral areas, which is the lesion area of the OIR model. Five days after the hADSC intravitreal injection, the area of neovascularization was reduced by 94.83% compared with that of the OIR group. Hematologic staining and electron microscopy did not show noticeable proliferation and degeneration of the retina.

Conclusions: This study provides evidence for the intraocular application of hADSCs.

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氧诱导脂肪源性间充质干细胞视网膜病变的体外诱导及眼内应用。

目的:我们设计了一项研究，旨在寻找人脂肪间充质干细胞(hADSCs)在眼内应用中的诱导、运动、融合、增殖和安全性的理论证据。方法:体外诱导hascs表达内皮细胞(ECs)特征。将HADSCs眼内注射到氧致视网膜病变(OIR)小鼠体内，观察其在体内的运动、融合、增殖和预后。电镜检查视网膜变化，确认hscs眼内应用的安全性。结果:诱导后，hscs表达内皮细胞标志物血管性血液病因子(vWF)。在OIR小鼠玻璃体内注射后，hascs分布在视网膜上方。注射的细胞没有与视网膜融合，聚集在中央和外周区域，这是OIR模型的病变区域。玻璃体内注射hADSC 5 d后，新生血管面积较OIR组减少94.83%。血液学染色和电镜未见明显的视网膜增生和变性。结论:本研究为hscs的眼内应用提供了依据。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.