Prevalence of filarial parasites in field-caught mosquitoes in northwestern California.

Abstract

Mosquitoes were collected in Lake County, CA, in 2014 and tested using standard polymerase chain reaction for filarial parasite DNA. Filarial parasites were detected in 23 out of 1,008 total pools. DNA from Dirofilaria immitis, the parasite causing dog heartworm, was detected in Aedes increpitus (MIR=4.62), Aedes sierrensis (MIR=6.72), Anopheles freeborni (MIR=1.08), and Culex tarsalis (MIR=0.10). Setaria yehi, deer body worm, was detected in Ae. sierrensis (MIR=13.42), Anopheles franciscanus (MIR=0.55), An. freeborni (MIR=2.69), and Culex stigmatosoma (MIR=0.41). The avian parasite Splendidofilaria could not be identified to species but was detected in Cx. tarsalis (MIR=0.20). DNA was also detected for three unidentified filarial parasites in Culex. Filarial-positive pools spanned May-August, with Splendidofilaria earlier in the season and S. yehi later. For D. immitis, MIR tended to be highest in June, when the 130 HDU development threshold was reached. Interestingly, D. immitis was also detected prior to the HDU threshold, and D. immitis was not detected August-September, though HDU remained high enough for development. This suggests that there are other factors influencing dog heartworm transmission in the area.