Determination of The Response of Wild and Cultivated Tomato Genotypes to Some Disease and Pests by Molecular Markers

Gülnur Aydin, H. Aktaş
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引用次数: 1

Abstract

Fusarium oxysporum f. sp. lycopersici, Verticillium spp., and nematodes, as well as virus diseases that negatively affect production with limited chemical control cause significant losses in greenhouse tomato cultivation. The practical and effective side of controlling diseases is genetic control by breeding. Using a genomic approach for plant breeding is a more sustainable and effective way to control disease and pests. The development of the resistant line is improved by conventional breeding methods that can be conducted over a long period. However, molecular markers make these processes considerably shorter with identifying resistant individuals. In this study, 14 wild and 188 cultivated tomato genotypes have been tested against Fusarium oxysporum f. sp. radicis-lycopersici (FORL), Meloidogyne spp. (root-knot nematodes), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV), Tomato yellow leaf curl virus (TYLCV) and Verticillum dahliae Kleb. (Ve) diseases and pests with using MAS (Marker-Assisted Selection) technique. According to these results, it has been determined that the selected markers can be used effectively in breeding studies to determine the diseases mentioned above.
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用分子标记测定野生和栽培番茄基因型对某些病虫害的反应
番茄赤霉病、番茄红霉病、黄萎病、线虫病以及病毒病等对番茄生产产生负面影响,化学防治有限,造成温室番茄栽培的重大损失。控制疾病的实际和有效的一面是通过育种进行遗传控制。利用基因组方法进行植物育种是一种更可持续、更有效的防治病虫害的方法。抗性品系的发展是通过传统育种方法进行的,这种方法可以进行很长一段时间。然而,分子标记使鉴定耐药个体的过程大大缩短。本研究对14种野生番茄和188种栽培番茄基因型进行了番茄根结线虫(FORL)、根结线虫(Meloidogyne spp)、番茄斑点枯萎病毒(TSWV)、番茄花叶病毒(ToMV)、番茄黄卷叶病毒(TYLCV)和大丽黄萎病(Verticillum dahliae Kleb)的检测。(5)利用MAS(标记辅助选择)技术进行病虫害鉴定。根据这些结果,可以确定所选择的标记可以有效地用于育种研究,以确定上述疾病。
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