Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy

Abstract

Movement of cellulose synthase particles have so far been observed on the plant epidermis that are amenable to confocal imaging, yielding appreciable signal and resolution to observe small plasma membrane-localised particles. Presented here is a method, using airyscan confocal microscopy, that permits similar information to be obtained at depth within the developing protoxylem vessels of intact roots.