In Vitro Inhibitory Effects and Bioinformatic Analysis of Norfloxacin and Ofloxacin on Piroplasm

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2022-12-21 DOI:10.1007/s11686-022-00648-9

Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Ikuo Igarashi

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引用次数: 1

Abstract

Purpose

The in vitro inhibitory effect of two fluroquinolone antibiotics, norfloxacin and ofloxacin, was evaluated in this study on the growth of several Babesia and Theileria parasites with highlighting the bioinformatic analysis for both drugs with the commonly used antibabesial drug, diminazene aceturate (DA), and the recently identified antibabesial drugs, luteolin, and pyronaridine tetraphosphate (PYR).

Methods

The antipiroplasm efficacy of screened fluroquinolones in vitro and in vivo was assessed using a fluorescence-based SYBR Green I assay. Using atom Pair signatures, we investigated the structural similarity between fluroquinolones and the antibabesial drugs.

Results

Both fluroquinolones significantly inhibited (P < 0.05) the in vitro growths of Babesia bovis (B. bovis), B. bigemina, B. caballi, and Theileria equi (T. equi) in a dose-dependent manner. The best inhibitory effect for both drugs was observed on the growth of T. equi. Atom Pair fingerprints (APfp) results and AP Tanimoto values revealed that both fluroquinolones, norfloxacin with luteolin, and ofloxacin with PYR, showed the maximum structural similarity (MSS). Two drug interactions findings confirmed the synergetic interaction between these combination therapies against the in vitro growth of B. bovis and T. equi.

Conclusion

This study helped in discovery novel potent antibabesial combination therapies consist of norfloxacin/ofloxacin, norfloxacin/luteolin, and ofloxacin/PYR.

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诺氟沙星和氟沙星对梨形体的体外抑制作用及生物信息学分析

目的本研究评价了两种氟喹啉酮抗生素诺氟沙星和氧氟沙星对几种巴贝虫和泰勒虫寄生虫生长的体外抑制作用，并重点对这两种药物与常用的抗标签药物乙酰二甲烯（DA）和最近鉴定的抗标签药木犀草素进行了生物信息学分析，方法采用SYBR Green I荧光法测定筛选出的氟喹啉酮在体外和体内的抗凝血作用。利用原子对标记，我们研究了氟喹啉酮和抗标签药物之间的结构相似性。结果两种氟喹啉酮均能显著抑制（P <； 0.05）牛巴贝斯虫（B.bovis）、双联乳巴贝斯虫、卡巴利巴贝斯虫和马泰勒虫（T.equi）的体外生长呈剂量依赖性。两种药物对马生长的抑制作用最好。原子对指纹图谱（APfp）结果和AP Tanimoto值显示，氟喹啉酮，诺氟沙星与木犀草素，以及氧氟沙星与PYR，都显示出最大的结构相似性（MSS）。两种药物相互作用的发现证实了这些联合疗法对牛双歧杆菌和马T.equi体外生长的协同作用。结论本研究有助于发现由诺氟沙星/氧氟沙星、诺氟沙星-木犀草素和氧氟沙星/PYR组成的新型强效抗菌联合疗法。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.