Molecular and Classical identification of Staphylococcus aureus, Isolated from Iraqi Patients with Recurrent Tonsillitis

Abstract

Tonsillitis is an infection of the tonsils caused by one of several types of bacteria or viruses. Staphylococcus aureus are one of most common bacteria isolated from recurrent tonsillitis, its identification by classical methods takes 3-5 days as well as the accuracy does not reach an absolute degree. So current study aimed to diagnose S. aureus by classical and molecular diagnosis to increase the accuracy of the diagnosis. Two hundred and fifteen tonsil swabs were collected from Iraqi patients susceptible suffered from recurrent tonsillitis who attended clinics in major hospitals in Najafe/ Iraq. Classical diagnoses of S. aureus were done by bacterial cultures, biochemical test and Vitek system. While molecular diagnosis achieved via PCR technique The results of classical methods showed that 50 isolates recovered from (195) suspected patients were S. aureus , while molecular diagnoses showed that 45 isolates out of 50 diagnostic S. aureus via classical methods were S. aureus with significant difference (P≤ 0.01) . In conclusion, Molecular method is more sensitive in diagnoses of S. aureus than conventional testes in case of recurrent tonsillitis.