Protective effect and mechanism of low P50 haemoglobin oxygen carrier on isolated rat heart

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2022-05-12 DOI:10.1080/21691401.2021.2017947

Wentao Zhou, Shen Li, Shasha Hao, Honghui Zhang, Tao Li, Wanjing Li, Jiaxin Liu, Hong Wang, Chengmin Yang

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Abstract

Abstract The protection of the isolated heart is very important in heart transplantation surgery, meanwhile, the ischaemia/reperfusion (I/R) of the isolated heart is the main cause of its damage. A timely supply of oxygen can significantly improve the prevention of myocardial ischaemia, however, the cardioprotective solution does not have an oxygen supply function. Haemoglobin Based on Oxygen Carriers (HBOCs) is a kind of nano-oxygen drug, which can effectively and timely supply oxygen to hypoxic organs and tissues. However, the oxygen-carrying and releasing capacity (P50) is different with different HBOCs. The aim of our study was to investigate whether STS (a kind of cardioprotective solution, St Thomas Solution) +different P50 HBOCs provide superior myocardial protection and decrease myocardial injury compared to only STS in rats Langendorff isolated heart perfusion model. The results showed that STS + HBOCs can improve cardiac function at 37 °C for 35 min and 120 min, and reduce myocardial infarctions, pathological changes, and apoptosis of cardiomyocytes, and the STS + low P50 HBOCs is more effective than the other two higher P50 HBOCs. We further demonstrated the outstanding protective effect of STS + low P50 HBOCs on cardiac function, reducing myocardial infarctions and apoptosis of cardiomyocytes in rat Langendorff isolated heart perfusion model.

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低P50血红蛋白氧载体对离体大鼠心脏的保护作用及机制

摘要离体心脏的保护在心脏移植手术中非常重要，而离体心脏的缺血/再灌注(I/R)是造成离体心脏损伤的主要原因。及时供氧可显著提高心肌缺血的预防效果，但保心液不具有供氧功能。基于氧载体的血红蛋白(HBOCs)是一种纳米氧药物，可以有效、及时地为缺氧器官和组织提供氧气。但不同hboc的携氧释放能力(P50)不同。本研究旨在探讨在Langendorff离体心脏灌注模型大鼠中，STS(一种心脏保护液，St Thomas溶液)+不同P50 hboc是否比单独使用STS具有更好的心肌保护和减轻心肌损伤的作用。结果表明，STS + HBOCs在37℃作用35 min和120 min时可改善心功能，减少心肌梗死、病理改变和心肌细胞凋亡，且STS +低P50 HBOCs比其他两种高P50 HBOCs效果更好。在Langendorff离体心脏灌注模型大鼠实验中，我们进一步证实了STS +低P50 hboc对心功能、心肌梗死和心肌细胞凋亡的显著保护作用。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.