Revisiting the high-fat diet/low streptozotocin prediabetic rat model: A bioanalytical adjustment

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY Journal of pharmacological and toxicological methods Pub Date : 2023-03-01 DOI:10.1016/j.vascn.2023.107252
Alejandra M. Preciado-Saldaña , José A. López-Díaz , J. Abraham Domínguez-Avila , J. Fernando Ayala-Zavala , Humberto F. Astiazaran-García , Gustavo A. González-Aguilar , Abraham Wall-Medrano
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Abstract

Insulin resistance (IR) is the main feature of prediabetes (PD), which ultimately leads to diabetes. High-dose streptozotocin-treated rodents often show irreversible β-cell mass loss and function, leaving the premorbid diabetic state (PD/IR) unnoticed. This study aimed to re-evaluate the synergistic/independent effect of a sub-chronic consumption (1–5 weeks) of a high-fat diet (60% gross energy from fat, 3.8 kcal.g−1) with [PD/IR-2 (week 2) to PD/IR-5 week five)] or without [HFD-5 (week five)] a single intraperitoneal dose (35 mg.kg−1) of streptozotocin in Wistar rats. Bioassay performance and clinical/histological features suggesting PD/IR or diabetes, were documented weekly and compared to standard chow-fed (3.5 kcal.g−1) rats (healthy controls, HC). PD/IR1–5 (fed with HFD for 1 to 5 weeks plus a single dose of streptozotocin) and HFD-5 (just fed with HFD for 5 weeks) groups reduced their food intake yet gained more body weight than HC. Groups exhibited hyperglycemia, dyslipidemia, and impaired glucose tolerance in decreasing order as follows: PD/IR-5, PD/IR-4, HFD-5, PD/IR-2-3, and HC. Histological disturbances in the pancreas, Soleus muscle, and liver were mostly observed in HFD-5 and PD/IR4–5 groups. HFD administration for 4 weeks white a single moderate dose of streptozotocin four days before sacrifice, leads to a convenient PD/IR rat model.

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重新审视高脂肪饮食/低链脲佐菌素糖尿病前期大鼠模型:生物分析调整
胰岛素抵抗(IR)是糖尿病前期(PD)的主要特征,最终导致糖尿病的发生。高剂量链脲佐菌素治疗的啮齿动物经常表现出不可逆的β细胞质量损失和功能,而不引起发病前糖尿病状态(PD/IR)的注意。本研究旨在重新评估Wistar大鼠亚慢性摄入高脂肪饮食(60%总能量来自脂肪,3.8 kcal.g−1)[PD/IR-2(第2周)至PD/IR-5(第5周)]或不服用[HFD-5(第5周)]单次腹腔注射链脲佐菌素(35 mg.kg−1)的协同/独立效应。每周记录提示PD/IR或糖尿病的生物测定性能和临床/组织学特征,并与标准周饲(3.5 kcal.g−1)大鼠(健康对照,HC)进行比较。PD/ IR1-5组(用HFD加单剂量链脲佐菌素喂养1至5周)和HFD-5组(只用HFD喂养5周)减少了食物摄入量,但体重却比HC组增加得更多。各组表现出高血糖、血脂异常和糖耐量下降的顺序如下:PD/IR-5、PD/IR-4、HFD-5、PD/IR-2-3和HC。HFD-5和PD/ IR4-5组胰腺、比目鱼肌和肝脏的组织学紊乱最为明显。HFD给药4周,献祭前4天给予单次中剂量链脲佐菌素,建立PD/IR大鼠模型。
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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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