Molecular Characterization of Mitochondrial Cytochrome c oxidase subunit 1 (Cox1) gene from Trichostrongylus Species (Nematoda: Trichostrongylidae) in Northern Iran

Q3 Medicine Turkiye parazitolojii dergisi Pub Date : 2023-03-02 DOI:10.4274/tpd.galenos.2022.16878

Meysam Sharifdini, Elham Hajialilo, Hedayat Hosseinnezhad, Mohammad Ali Mohammadi

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Abstract

Objective: The objective of the present study was to identify Trichostrongylus species by molecular analysis and also phylogenetic relationships of Trichostrongylus species by mitochondrial Cytochrome c oxidase subunit 1 (Cox1) gene in Guilan province, northern Iran.

Methods: Abomasum and duodenum contents of 144 livestock were collected from sheep, goats, and cattle in Guilan province. Morphological survey was performed for initial screening. Total DNA was extracted, and the partial region of Cox1 gene was amplified and sequenced. Genetic diversity was calculated and phylogenetic analysis of the data on nucleotide sequence was conducted by MEGA7 software.

Results: Three species of Trichostrongylus including T. colubriformis, T. vitrinus, and T. axei were identified by morphological characteristics. The genetic divergence within the species in the present study was observed for T. axei (0-2.5%), T. colubriformis (0.77%), and T. vitrinus (0%). The mean inter-species difference between the three species of Trichostrongylus obtained in this study was 14.4-15.4%.

Conclusion: The Cox1 sequences of the members of Trichostrongylus spp. were highly variable and this could be used as a valuable measure to achieve a proper assessment on biodiversity. Sequence data generation from other species of Trichostrongylus will be needed to reconstruct the phylogenetic relationships of this genus of nematodes.

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伊朗北部毛线虫种线粒体细胞色素c氧化酶亚基1 (Cox1)基因的分子特征

目的:通过分子分析鉴定伊朗北部桂兰省的毛线虫种类，并利用线粒体细胞色素c氧化酶亚基1 (Cox1)基因进行毛线虫种间的系统发育关系。方法:采集桂兰地区绵羊、山羊、牛等144只家畜的皱胃和十二指肠内容物。进行形态学调查进行初步筛选。提取总DNA，扩增部分Cox1基因序列。利用MEGA7软件计算遗传多样性，并对核苷酸序列数据进行系统发育分析。结果:通过形态特征鉴定出了3种毛线虫，分别为色状毛线虫、玻璃状毛线虫和轴状毛线虫。在本研究中发现的种内遗传差异主要表现为:轴尾绦虫(0-2.5%)、色状绦虫(0.77%)和玻璃状绦虫(0%)。本研究获得的3种毛线虫种间平均差异为14.4 ~ 15.4%。结论:毛线虫成员的Cox1序列具有较高的多样性，可作为生物多样性评价的重要指标。从其他种类的毛线虫的序列数据生成将需要重建该属线虫的系统发育关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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