Topical application of indigo-plant leaves extract enhances healing of skin lesion in an excision wound model in rats.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Journal of applied biomedicine Pub Date : 2022-12-01 DOI:10.32725/jab.2022.014

Mohamed A Saleh, Ahmed A Shabaan, Michel May, Youssif M Ali

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Abstract

Objectives: This study aims to evaluate the pharmacological role of indigo extract in accelerating the wound healing in a rat model.

Methods: Female Sprague-Dawley rats were anesthetized with ketamine (30 mg/kg, i.p.) and the full thickness of the marked skin was then cut carefully and wounds were left undressed. Indigo extract (5%) in PBS was applied topically twice daily until healing was complete. A control group of rats was treated with povidone-iodide (Betadine®). Rats treated with phosphate buffer saline were used as a negative control group. The rate of wound healing was assessed daily. Histopathological examination of skin sections were qualitatively assessed by independent evaluators. The inflammatory and apoptotic markers were assessed in skin tissue homogenates using ELISA.

Results: Histopathology data showed that applying indigo to skin wounds enhanced the healing process, resulting in a significant decrease in dermal inflammation in comparison to untreated rats. Topical application of indigo significantly increased antioxidant enzyme activities with reduced malondialdehyde (MDA) levels in wound tissues. The levels of matrix metalloproteases-2 and -9 were significantly lower with an accompanied increase in the level of TGF-β1 in skin tissues from rats treated with indigo compared to the control group treated with PBS.

Conclusions: The antioxidant and anti-inflammatory properties of indigo leaf extract accelerate the healing of skin injuries.

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局部应用靛蓝植物叶提取物促进大鼠皮肤损伤的愈合。

目的:研究靛蓝提取物对大鼠创面愈合的促进作用。方法:雌性Sprague-Dawley大鼠用氯胺酮(30mg /kg, i.p)麻醉后，仔细切开标记皮肤全层，剥去创面。靛蓝提取物(5%)在PBS中局部应用，每天两次，直到愈合完全。对照组大鼠给予碘聚维酮(倍他定®)治疗。以磷酸缓冲盐水处理大鼠为阴性对照组。每日评估创面愈合率。皮肤切片的组织病理学检查由独立评估人员进行定性评估。采用ELISA法检测皮肤组织匀浆中的炎症和凋亡标志物。结果:组织病理学数据显示，与未处理的大鼠相比，将靛蓝涂抹在皮肤伤口上可以促进愈合过程，导致皮肤炎症显著减少。靛蓝外用可显著提高伤口组织抗氧化酶活性，降低丙二醛(MDA)水平。与PBS处理组相比，靛蓝处理组大鼠皮肤组织中基质金属蛋白酶-2和-9水平明显降低，TGF-β1水平升高。结论:靛蓝叶提取物具有抗氧化和抗炎作用，能促进皮肤损伤的愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.