Cloning and Tissue-specific Expression of Spliced Variants of the Rat Organic Anion Transporter (rOAT-K)

M. Wolff, T. Su
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引用次数: 1

Abstract

During the last decade, molecular cloning has identified several families of multispecific organic ion transporters mediating the renal and hepatic elimination of organic ions. Clinically, these transporters play important roles in the renal tubular secretion and reabsorption of various drugs. They are also in part responsible for the drug pharmacologic responses, drug-drug interactions, and drug nephrotoxicity. This study describes 12 novel isoforms of the rat kidney organic anion transporter rOAT-K. These isoforms are spliced variants of the same gene arising from alternative splicing of six regions defined as A-F. The two previously reported isoforms rOAT-K1 and rOAT-K2 were also found to be spliced variants of this gene. The open reading frames of the 12 isoforms encode a range of 352-670 amino acid proteins. Tissue distribution studies showed that the majority of the isoforms are kidney- and liver-specific.
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大鼠有机阴离子转运蛋白(rOAT-K)剪接变体的克隆及组织特异性表达
在过去的十年中,分子克隆已经确定了几个多特异性有机离子转运体家族,介导肾脏和肝脏的有机离子消除。临床上,这些转运蛋白在各种药物的肾小管分泌和重吸收中起重要作用。它们也部分负责药物药理反应、药物-药物相互作用和药物肾毒性。本研究描述了大鼠肾有机阴离子转运蛋白rOAT-K的12种新亚型。这些同工异构体是同一基因的剪接变体,由定义为A-F的六个区域的交替剪接产生。先前报道的两个同种异构体rOAT-K1和rOAT-K2也被发现是该基因的剪接变体。12种同种异构体的开放阅读框编码了352-670个氨基酸蛋白。组织分布研究表明,大多数亚型是肾脏和肝脏特异性的。
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