High Through-Put Parallel Biomolecules Sorting Microsystem with Three Dimensional PDMS Stack

Y. Nara, H. Sugino, T. Arakawa, Y. Shirasaki, T. Funatsu, S. Shoji
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引用次数: 1

Abstract

High through-put parallel biomolecules sorter was fabricated with the three dimensional PDMS stack structure. Three dimensional channel structure was realized by using PDMS dry etching. High speed switching of 10 ms order in each channel was realized with four and eight parallel type sorter. Sorting rate is about 20 samples/sec in the case of the four parallel type sorter. High through-put sorting of about 20 times higher than that of the previous single channel sorter was obtained in actual E. coli cells separation.
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具有三维PDMS堆栈的高通量平行生物分子分选微系统
采用三维PDMS堆叠结构制备了高通量平行生物分子分选机。采用PDMS干法刻蚀实现了三维通道结构。采用4路和8路并行分选器,实现了每路10ms顺序的高速切换。在四并联式分选机的情况下,分选速率约为20个样品/秒。在实际的大肠杆菌细胞分离中,获得了比以往单通道分选机高20倍左右的高通量分选。
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