Mapping Cell Phenomics with Multiparametric Flow Cytometry Assays.

IF 3.7 Q2 GENETICS & HEREDITY Phenomics (Cham, Switzerland) Pub Date : 2022-01-09 eCollection Date: 2022-08-01 DOI:10.1007/s43657-021-00031-0

Yang Liu, Haichu Zhao, Boqiang Fu, Shan Jiang, Jing Wang, Ying Wan

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Abstract

Phenomics explores the complex interactions among genes, epigenetics, symbiotic microorganisms, diet, and environmental exposure based on the physical, chemical, and biological characteristics of individuals and groups. Increasingly efficient and comprehensive phenotyping techniques have been integrated into modern phenomics-related research. Multicolor flow cytometry technology provides more measurement parameters than conventional flow cytometry. Based on detailed descriptions of cell phenotypes, rare cell populations and cell subsets can be distinguished, new cell phenotypes can be discovered, and cell apoptosis characteristics can be detected, which will expand the potential of cell phenomics research. Based on the enhancements in multicolor flow cytometry hardware, software, reagents, and method design, the present review summarizes the recent advances and applications of multicolor flow cytometry in cell phenomics, illuminating the potential of applying phenomics in future studies.

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利用多参数流式细胞仪测定绘制细胞表型组学图

表型组学根据个体和群体的物理、化学和生物特征，探索基因、表观遗传学、共生微生物、饮食和环境暴露之间复杂的相互作用。现代表型组学相关研究已融入了越来越高效和全面的表型技术。与传统的流式细胞仪相比，多色流式细胞仪技术可提供更多的测量参数。基于对细胞表型的详细描述，可以区分稀有细胞群和细胞亚群，发现新的细胞表型，检测细胞凋亡特征，这将拓展细胞表型组学研究的潜力。基于多色流式细胞仪硬件、软件、试剂和方法设计的改进，本综述总结了多色流式细胞仪在细胞表型组学中的最新进展和应用，阐明了表型组学在未来研究中的应用潜力。

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Phenomics (Cham, Switzerland)

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