The Expression of pmr A gene in Acinetobacter baumannii Bacteria that Responsible for Colistin Resistance

Abstract

A total of Forty isolates of Acinetobacter baumannii was Collected from many Hospitals (Teaching Baghdad Hospital, Teaching Pediatric Hospital, Al-Shahid Kazy Al-Harery and teaching clinical laboratories, Al-Yarmok hospital and Al-Kadhimia hospital) in Baghdad at the period 15/11/2018 to 19/2/2019, including sputum 15 isolates (37.5%), Blood 14 isolates (53%), wound swab 4 isolates (15%), Urine 4 isolates (10%), Burns one isolate (2.5%), pleural effusion fluids one isolate (2.5%) and throat swab one isolate (2.5%) . The current study showed that six isolates of A. baumannii that were resistant to colistin (polymyxin) carry the ( pmrA ) gene at product size was 175bp. The gene expression of the pmr A gene for bacteria was measured once it was treated with a Colistin (polymyxin) antibiotic at a concentration (<=0.5µl) of 0.48 ml and without treated by using MIC (minimum inhibitory concentration) method as it was measured. It was observed that there was no increase in the gene expression of pmrA gene after the bacterial isolate was treated with Colistin (polymyxin