Detection of antigen-specific human serum proteins related to the T-cell receptor in infectious disease and in an immune response to milk proteins or chemicals.

C. Little, G. Georgiou, G. Fey, B. Ravindran, J. Levine, H. Ogedegbe, H. Yamase, R. Cone
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引用次数: 1

Abstract

A monoclonal IgG2 antibody, MG3C9-1 A12, was prepared by immunization of mice with human serum Cohn Fraction III proteins enriched for TCR Ca+ proteins. MG3C9-1 A12 bound to Mr 28,000, antigen-specific TCR Ca+, beta-, and TCR Ca+, beta+ serum proteins associated with TGF-beta1, 2. The IgG2 monoclonal antibody also bound to T-lymphocyte proteins but did not bind to B lymphocyte proteins, human albumin, IgM, IgG, IgA, or TGF-beta1, 2, 3 immunogenic peptides. Monoclonal MG3C9-1 A12 detected TCR-related proteins specific for filarial extract, milk proteins, or benzoic acid in the sera of individuals with chronic or asymptomatic filariasis, milk intolerance, or sensitivity to toluene, respectively. TCR-related serum proteins were also detected intracellularly in mononuclear cells in frozen sections of ileum from a patient with milk intolerance and reactive mesenteric lymph nodes from a patient with a gastric ulcer. The results suggest that antigen-specific TCR-related serum proteins may be elevated during an immune response to oral, environmental, or infectious stimuli.
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在传染病和对牛奶蛋白或化学物质的免疫反应中与t细胞受体相关的抗原特异性人血清蛋白的检测。
用富含TCR Ca+蛋白的人血清Cohn Fraction III蛋白免疫小鼠制备IgG2单克隆抗体MG3C9-1 A12。mg3c9 - 1a12结合Mr 28000,抗原特异性TCR Ca+, β -和TCR Ca+, β +血清蛋白与tgf - β a1, 2。IgG2单克隆抗体也与t淋巴细胞蛋白结合,但不与B淋巴细胞蛋白、人白蛋白、IgM、IgG、IgA或tgf - β 1,2,3免疫原性肽结合。单克隆MG3C9-1 A12分别检测慢性或无症状丝虫病、牛奶不耐受或甲苯敏感患者血清中丝虫病提取物、牛奶蛋白或苯甲酸特异性tcr相关蛋白。在一名牛奶不耐受患者的回肠冷冻切片和一名胃溃疡患者的反应性肠系膜淋巴结中,也检测到细胞内单个核细胞中的tcr相关血清蛋白。结果表明抗原特异性tcr相关血清蛋白可能在口腔、环境或感染刺激的免疫反应中升高。
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