Improvement of Phenolic Compound Extraction by Using Ion Exchange Chromatography and Evaluation of Biological Activities of Polyphenol-enriched Fraction of Rosa canina Fruits.

IF 1.8 4区医学 Q3 PHARMACOLOGY & PHARMACY Iranian Journal of Pharmaceutical Research Pub Date : 2022-12-01 DOI:10.5812/ijpr-126558

Zahra Sabahi, Seyed Muhammad Farid Hasan, Seyed Abdulmajid Ayatollahi, Fatemeh Farmani, Afshin Afsari, Mahmoodreza Moein

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引用次数: 1

Abstract

Background: Rosa canina has been traditionally known as a medicinal plant. Different applications of fruits (Rose hip) comprise the food, perfume, and cosmetic industries.

Objectives: This study aimed to prepare an enriched polyphenolic fraction from Rosa canina in addition to its biological activities.

Methods: Poly phenolic enriched fraction was prepared using Amberlite XAD-7 for removing unwanted components. Phenols, flavonoids, and anthocyanins content analyses showed that they increased significantly compared to the extract. HPLC analysis showed that this fraction is a rich source of ascorbic acid.

Results: The results of the DPPH, ferric-reducing antioxidant power (FRAP), ABTS, and nitric oxide assay confirmed that the antioxidant activities of the fraction had been increased compared to the extract. The oxygen radical absorbance capacity (ORAC) assay and cellular antioxidant activity of the fraction also confirmed its potential antioxidant activity. This fraction showed xanthine oxidase inhibitory activity at 100 µg/mL concentration. Comet assay analysis revealed that this fraction at 25 - 100 µg/mL concentrations inhibited H₂O₂ genotoxicity in human lymphocytes.

Conclutions: This study suggests that the fruit of Rosa canina could be considered as a potential antioxidant, a xanthine oxidase inhibitor, and an antigenotoxic source, and the application of Amberlite XAD-7 improves extraction efficiencies through enrichment of phenolic compounds in this plant.

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离子交换色谱法提取多酚类化合物的工艺改进及富多酚部分的生物活性评价。

背景:Rosa canina传统上被认为是一种药用植物。水果(玫瑰果)的不同用途包括食品、香水和化妆品行业。目的:制备具有丰富生物活性的Rosa canina多酚提取物。方法:用Amberlite XAD-7制备多酚富集组分，去除多余成分。酚类、黄酮类和花青素含量分析表明，与提取物相比，它们的含量显著增加。高效液相色谱分析表明，该部分是抗坏血酸的丰富来源。结果:DPPH、铁还原抗氧化能力(FRAP)、ABTS和一氧化氮测定结果证实，该部位的抗氧化活性较提取物有所提高。氧自由基吸收能力(ORAC)测定和细胞抗氧化活性也证实了其潜在的抗氧化活性。在100µg/mL浓度下，黄嘌呤氧化酶抑制活性显著。彗星分析显示，该组分在25 - 100µg/mL浓度下抑制H2O2对人淋巴细胞的遗传毒性。结论:本研究提示，玫瑰果实可作为潜在的抗氧化剂、黄嘌呤氧化酶抑制剂和抗毒素源，而Amberlite XAD-7的应用可通过富集该植物中的酚类化合物提高提取效率。

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来源期刊

Iranian Journal of Pharmaceutical Research 医学-药学

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

2 months

期刊介绍： The Iranian Journal of Pharmaceutical Research (IJPR) is a peer-reviewed multi-disciplinary pharmaceutical publication, scheduled to appear quarterly and serve as a means for scientific information exchange in the international pharmaceutical forum. Specific scientific topics of interest to the journal include, but are not limited to: pharmaceutics, industrial pharmacy, pharmacognosy, toxicology, medicinal chemistry, novel analytical methods for drug characterization, computational and modeling approaches to drug design, bio-medical experience, clinical investigation, rational drug prescribing, pharmacoeconomics, biotechnology, nanotechnology, biopharmaceutics and physical pharmacy.