Allergen bronchoprovocation: correlation between FEV1 maximal percent fall and area under the FEV1 curve and impact of allergen on recovery.

Abstract

Background: House dust mite (HDM) induces greater responses than other allergens during allergen bronchoprovocation (ABP) testing. The two standardized methods for reporting results of ABP tests are the maximal percent fall in forced expiratory volume in one second (FEV_{1, max}; %) and the area under the FEV₁ vs time curve (AUC; %FEV₁ x min). The relationship between these methods has not been previously investigated.

Aims: We aimed to measure the correlation between FEV_{1, max} and AUC during the early asthmatic response (EAR) and the late asthmatic response (LAR), and to determine if the EAR recovery period for HDM would be longer than other allergens (cat, grass, horse, and ragweed).

Methods: We retrospectively calculated the AUC and correlation between FEV_{1, max} and AUC during the EAR_(0-2 h) and LAR_(3-7 h) for each allergen. We compared EAR_(0-3 h) and LAR_(3-7 h) FEV_{1, max}, AUC and absolute difference in FEV_{1, max} to the most recovered FEV₁ (FEV_{1, min}). We performed pairwise comparisons of correlation and slope values using Fischer's r to z transformation and t-tests, respectively. AUC and absolute differences in FEV_{1, max} and FEV_{1, min} were compared using a one-way ANOVA test, followed by a post-hoc Scheffe test.

Results: Correlation between the FEV_{1, max} and AUC during the EAR_(0-2 h) (n = 221) was 0.807, and was 0.798 during the LAR_(3-7 h) (n = 157 of 221), (difference p = 0.408). The EAR_(0-3 h) AUC and FEV_{1, max} did differ between allergens (both p < 0.0001) but the LAR_(3-7 h) AUC and FEV_{1, max} did not (p = 0.548 and 0.824, respectively). HDM did not have a larger AUC or FEV_{1, max}, than all other allergens during the EAR_(0-3 h) or the LAR_(3-7 h). The absolute difference between the FEV_{1, max} and FEV_{1, min} during the EAR_(0-3 h) did not differ between allergens (p = 0.180).

Conclusion: The FEV_{1, max} and AUC for both the EAR_(0-2 h) and LAR_(3-7 h) had excellent correlation, with no significant difference. Thus, significant bronchoconstriction will likely result in a longer recovery period. There was no evidence of delayed EAR_(0-3 h) recovery following HDM challenges, so HDM did not induce a larger response compared to all the other allergens examined.

Registration: Not registered. This is not a clinical trial. (This study is a retrospective analysis of data collected during several registered trials.).