Studies on a barley mutant lacking chlorophyl b. I. Photochemical activity of isolated chloroplasts

N.K. Boardman, H.K. Highkin
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引用次数: 105

Abstract

  • 1.

    1. Chloroplasts were isolated from the leaves of a barley mutant which lacked chlorophyll b and compared with the chloroplasts from the normal strain which had the normal content of chlorophyll a and chlorophyll b.

  • 2.

    2. Hill-reaction measurements were made with either 2,3′,6-trichlorophenol-indophenol, ferricyanide or NADP+ as oxidant. At a light intensity of 40 000 lux, the mutant chloroplasts were more active per mg of total chlorophyll than the normal chloroplasts, but only slightly more active per mg of chlorophyll a. Normal chloroplasts were saturated at a light intensity of about 30 000 lux whereas the mutant chloroplasts required an intensity of nearly 60 000 lux for saturation. At low light intensities (< 15 000 lux) the mutant chloroplasts were less active than the normal chloroplasts. The mutant chloroplasts were inactivated at a greater rate if allowed to stand at 0° and they were more susceptible to inhibition by 1-(p-chlorophenyl)-3,3′-dimethylurea.

  • 3.

    3. Microscopic examination under phase contrast showed no significant differences in the appearance of isolated chloroplasts from the normal and mutant plants.

    The lack of chlorophyll b in the mutant was confirmed by absorption and spectrofluorimetric measurements at the temperature of liquid nitrogen. The spectrofluorimetric method was capable of detecting one molecule of chlorophyll b in the presence of 1000 molecules of chlorophyll a.

  • 4.

    4. The results are discussed in relation to the function of chlorophyll b in photosynthesis.

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大麦缺叶绿素突变体的研究。ⅰ。离体叶绿体的光化学活性
1.1. 从一个缺乏叶绿素b的大麦突变体叶片中分离出叶绿体,并与叶绿素a和叶绿素b含量正常的正常菌株进行了比较。用2,3 ',6-三氯苯酚-吲酚、铁氰化物或NADP+作为氧化剂进行希尔反应测定。在40000勒克斯的光强下,突变体叶绿体每毫克总叶绿素的活性高于正常叶绿体,但每毫克叶绿素a的活性仅略高于正常叶绿体。正常叶绿体在约30000勒克斯的光强下饱和,而突变体叶绿体需要近60000勒克斯的光强才能饱和。在低光强度下(<15 000勒克斯)时,突变体的叶绿体活性低于正常叶绿体。在0°温度下,突变体的失活率更高,更容易受到1-(对氯苯)-3,3′-二甲基脲的抑制。相衬显微镜检查显示,正常植株和突变植株分离的叶绿体外观没有显著差异。在液氮温度下的吸收和荧光光谱测量证实了突变体中叶绿素b的缺乏。荧光光谱法能够在1000个叶绿素a.4.4存在的情况下检测到1个叶绿素b分子。并就叶绿素b在光合作用中的作用进行了讨论。
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