{"title":"⿿Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples","authors":"Tue Bjerg Bennike , Kenneth Kastaniegaard , Simona Padurariu , Michael Gaihede , Svend Birkelund , Vibeke Andersen , Allan Stensballe","doi":"10.1016/j.euprot.2015.10.001","DOIUrl":null,"url":null,"abstract":"<div><p>Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described.</p><p>Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30<!--> <!-->min. The protein content of the samples was characterized by high throughput quantitative proteomics.</p><p>We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor differences in protein quantitation.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"10 ","pages":"Pages 9-18"},"PeriodicalIF":0.0000,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.10.001","citationCount":"40","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515300222","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 40
Abstract
Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described.
Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30 min. The protein content of the samples was characterized by high throughput quantitative proteomics.
We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor differences in protein quantitation.
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