Oligonucleotide-directed mutagenesis using plasmid DNA: a screening step to confirm the first successful step to positive mutagenesis

Abstract

The technique of site-directed mutagenesis (SDM) is widely used in molecular biology to introduce mutations into DNA (Ref. [1]). This article discusses a quick screening step that can be incorporated into mutagenesis systems such as the {GeneEditor in vitro site-directed mutagenesis system (Promega, Madison, WI, USA)}, which manipulates target DNA cloned into an Escherichia coli plasmid and uses antibiotic selection to select for mutants.