252Cf-plasma desorption mass spectrometry of covalently bound nucleic acid adducts: Psoralen-nucleosides photoadducts

International Journal of Mass Spectrometry and Ion Physics Pub Date : 1983-09-20 DOI:10.1016/0020-7381(83)85103-1

P. Vigny , M. Spiro , F. Gaboriau , Y. Le Beyec , S. Della Negra , J. Cadet , L. Voituriez

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引用次数: 15

Abstract

Psoralen-nucleosides photocycloadducts have been studied by ²⁵²Cf-Plasma Desorption Mass Spectrometry. The present study involves isolation, high performance liquid chromatography purification and mass spectrometry analysis of the two major monoaddition products formed in the photoreaction of the naturally occurring 8-methoxypsoralen with thymidine. Structural informations have also been obtained concerning photocycloadducts of a new linear psoralen derivative 3-carbethoxypsoralen with thymidine and cytidine. A photobinding of 3-carbethoxypsoralen with adenosine has also been studied. The mass spectra of the adducts have been obtained directly without any derivatization. In all cases they showed molecular and quasimolecular ions in the positive and/or negative mode. It is concluded that ²⁵²Cf-PDMS is a powerful and easy technique for studying xenobiotic-modified nucleic acids.

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共价结合核酸加合物的等离子解吸质谱分析:补骨脂素核苷光加合物

用252cf等离子体解吸质谱法研究了补骨脂素核苷光环加合物。本研究对天然存在的8-甲氧基补骨脂素与胸腺嘧啶光反应形成的两种主要单加成产物进行了分离、高效液相色谱纯化和质谱分析。还获得了一种新的胸腺嘧啶和胞苷线性补骨脂素衍生物3-碳氧基补骨脂素的光环加合物的结构信息。还研究了3-碳氧基补骨脂素与腺苷的光结合。加合物的质谱直接得到，没有任何衍生化。在所有情况下，它们都显示出正或负模式的分子和准分子离子。结果表明，252Cf-PDMS技术是研究外源修饰核酸的一种简便易行的技术。

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International Journal of Mass Spectrometry and Ion Physics

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