Phytochemical investigation, antioxidant and anticancer activities of various Unani drugs.

Q2 Pharmacology, Toxicology and Pharmaceutics Drug metabolism and personalized therapy Pub Date : 2023-03-01 DOI:10.1515/dmpt-2022-0110

Mohd Nazir Mannan, Lahari Kuna, Alokananda Chakraborty, Mohammad Zakir, Syeda Hajra Fatima, Ahmed Minhajuddin, Munawwar Husain Kazmi, Tasleem Ahmad

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引用次数: 1

Abstract

Objectives: To analyze the phytochemicals, antioxidant, and anticancer activities on MCF-7 human breast cancer cell line using aqueous, hydro-ethanol, and methanol extracts of different Unani drugs, e.g., Halela Siyah, Aftimoon, Bisfayej, Ustukhudoos, and Kutki.

Methods: The qualitative examination (alkaloids, terpenoids, tannins, and saponins), anticancer activity, and an antioxidant assay of the three different extracts were done by MTT assay and DPPH assay, respectively, using different Unani drugs.

Results: The qualitative examination confirmed the substantive presence of phytochemical constituents in all the extracts of these drugs. The Methanolic extract of Halela Siyah had the highest DPPH scavenging activity (91%), while Bisfayej had the lowest (58%). Similarly, the hydro-ethanolic extract showed approximately identical activity for Halela Siyah (89%), Aftimoon (88%), Bisfayej (84%), Kutki (82%), and Ustukhudoos (81%). The aqueous extracts of Halela Siyah (88%) had the highest DPPH scavenging activity, whereas Bisfayej (73%) had the lowest. The methanolic extract of Aftimoon demonstrated the greatest anticancer activity (IC₅₀ - 108), while Aftimoon showed the least activity (IC₅₀ - 316). Halela Siyah (IC₅₀ - 175) and Aftimoon (IC₅₀ - 178) showed substantially the same activity in aqueous extracts. Ustukhudoos hydro-ethanol extracts had the highest (IC₅₀ - 130) activity, whereas Aftimoon had the lowest (IC₅₀ - 204).

Conclusions: In conclusion, our findings evaluated the presence of phytochemicals, good antioxidant activity, and anticancer activity in different extracts of drugs used in this study. The study shows these drugs have potential anticancer activity against breast cancer in MCF-7 cell lines.

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植物化学研究、抗氧化和抗癌活性。

目的:分析不同乌拉尼药物(Halela Siyah, Aftimoon, Bisfayej, Ustukhudoos, Kutki)的水、水乙醇和甲醇提取物对MCF-7人乳腺癌细胞株的植物化学成分、抗氧化和抗癌活性。方法:采用MTT法和DPPH法分别对三种不同提取物进行定性分析(生物碱、萜类、单宁、皂苷)、抗肿瘤活性和抗氧化活性测定。结果:定性分析证实，这些药物提取物中均含有大量植物化学成分。甲醇提取物对DPPH的清除活性最高(91%)，而Bisfayej最低(58%)。同样，水乙醇提取物对Halela Siyah(89%)、Aftimoon(88%)、Bisfayej(84%)、Kutki(82%)和Ustukhudoos(81%)的活性大致相同。水提物对DPPH的清除活性最高(88%)，而Bisfayej的清除活性最低(73%)。Aftimoon甲醇提取物的抗癌活性最高(IC50 ~ 108)， Aftimoon活性最低(IC50 ~ 316)。Halela Siyah (IC50 - 175)和Aftimoon (IC50 - 178)在水提液中的活性基本相同。水乙醇提取物的活性最高(IC50 ~ 130)， Aftimoon最低(IC50 ~ 204)。结论:总之，我们的研究结果评估了本研究中使用的药物的不同提取物中存在的植物化学物质，良好的抗氧化活性和抗癌活性。研究表明，这些药物对MCF-7细胞系的乳腺癌具有潜在的抗癌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug metabolism and personalized therapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

2.30

自引率

0.00%

发文量

期刊介绍： Drug Metabolism and Personalized Therapy (DMPT) is a peer-reviewed journal, and is abstracted/indexed in relevant major Abstracting Services. It provides up-to-date research articles, reviews and opinion papers in the wide field of drug metabolism research, covering established, new and potential drugs, environmentally toxic chemicals, the mechanisms by which drugs may interact with each other and with biological systems, and the pharmacological and toxicological consequences of these interactions and drug metabolism and excretion. Topics: drug metabolizing enzymes, pharmacogenetics and pharmacogenomics, biochemical pharmacology, molecular pathology, clinical pharmacology, pharmacokinetics and drug-drug interactions, immunopharmacology, neuropsychopharmacology.