The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart.

IF 1.2 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS Journal of Cardiovascular and Thoracic Research Pub Date : 2022-01-01 DOI:10.34172/jcvtr.2022.31599
Mitra Abdollahi-Diba, Jabar Bashiri, Hadi Pourmanaf, Vahid Fekri-Kourabbaslou
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Abstract

Introduction: Considering the effect of apoptosis on cardiovascular disease, this study aimed to determine the combined effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in the myocardium of male rats. Methods: A total of 35 male rats were randomly divided into five groups (n=7) as follows: endurance exercise+rosehip extract supplementation (Ex+Supp), endurance exercise (Ex), rosehip extract supplementation (Supp), six-month control (Con2), and three-month control (Con). The subjects in Ex+Supp and Ex groups performed endurance exercise (running on a treadmill at 24-33 m/min for 10-60 min) for 12 weeks, five times a week. Subjects in Ex+Supp and Supp groups consumed 1000 milligrams/ kilogram of rosehip extract for 12 weeks. Also, Con and Con2 groups did not receive any intervention. To RNA extraction and synthesis cDNA and evaluate the P53 and cytochrome C genes of the myocardium of rats, RT-PCR analysis was used. Results: Neither endurance exercise nor rosehip alone nor together significantly affected the expression of cytochrome C and P53 genes in the heart muscle of male rats (P>0.05). Also, endurance exercise (P=0.001) and rosehip supplementation (P=0.002) alone and in interaction (P<0.01) had a significant effect on body weight, myocardium weight, and the ratio of myocardium weight to body weight in male rats. Conclusion: Twelve weeks of endurance exercise accompanied with rosehip extract did not significantly affect the expression of P53 and cytochrome C genes. Further studies are suggested to confirm these results.

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耐力运动和补充玫瑰果提取物对雄性大鼠心脏P53和细胞色素C基因表达的影响。
导读:考虑到细胞凋亡对心血管疾病的影响,本研究旨在确定耐力运动和补充玫瑰果提取物对雄性大鼠心肌中P53和细胞色素C基因表达的联合影响。方法:将35只雄性大鼠随机分为5组(n=7),分别为耐力运动+补充玫瑰果提取物(Ex+Supp)、耐力运动(Ex) +补充玫瑰果提取物(Supp)、6个月对照组(Con2)和3个月对照组(Con)。Ex+Supp组和Ex组进行耐力运动(在跑步机上以24-33米/分钟的速度跑10-60分钟),为期12周,每周5次。Ex+Supp组和Supp组的受试者连续12周服用1000毫克/千克玫瑰果提取物。另外,Con组和Con2组没有接受任何干预。采用RT-PCR方法提取和合成cDNA,评价大鼠心肌组织中P53和细胞色素C基因的表达。结果:耐力运动和玫瑰果单独或共同作用均未显著影响雄性大鼠心肌细胞色素C和P53基因的表达(P>0.05)。此外,耐力运动(P=0.001)和补充玫瑰果(P=0.002)单独或相互作用(P)结论:12周耐力运动伴玫瑰果提取物对P53和细胞色素C基因的表达没有显著影响。建议进一步的研究来证实这些结果。
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来源期刊
Journal of Cardiovascular and Thoracic Research
Journal of Cardiovascular and Thoracic Research CARDIAC & CARDIOVASCULAR SYSTEMS-
CiteScore
2.00
自引率
0.00%
发文量
22
审稿时长
7 weeks
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