{"title":"Evaluation of the cell viability and antimicrobial effects of orthodontic bands coated with silver or zinc oxide nanoparticles: An <i>in vitro</i> study.","authors":"Rashin Bahrami, Maryam Pourhajibagher, Alireza Badiei, Reza Masaeli, Behrad Tanbakuchi","doi":"10.4041/kjod22.091","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>We aimed to evaluate the cell viability and antimicrobial effects of orthodontic bands coated with silver or zinc oxide nanoparticles (nano-Ag and nano-ZnO, respectively).</p><p><strong>Methods: </strong>In this experimental study, 30 orthodontic bands were divided into three groups (n = 10 each): control (uncoated band), Ag (silver-coated band), and ZnO (zinc oxide-coated band). The electrostatic spray-assisted vapor deposition method was used to coat orthodontic bands with nano-Ag or nano-ZnO. The biofilm inhibition test was used to assess the antimicrobial effectiveness of nano-Ag and nano-ZnO against <i>Streptococcus mutans</i>, <i>Lactobacillus acidophilus</i>, and <i>Candida albicans</i>. Biocompatibility tests were conducted using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The groups were compared using oneway analysis of variance with a post-hoc test.</p><p><strong>Results: </strong>The Ag group showed a significantly higher reduction in the number of <i>L. acidophilus</i>, <i>C. albicans</i>, and <i>S. mutans</i> colonies than the ZnO group (<i>p</i> = 0.015, 0.003, and 0.005, respectively). Compared with the control group, the Ag group showed a 2-log<sub>10</sub> reduction in all the microorganisms' replication ability, but only <i>S. mutants</i> showed a 2-log<sub>10</sub> reduction in replication ability in the ZnO group. The lowest mean cell viability was observed in the Ag group, but the difference between the groups was insignificant (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>Coating orthodontic bands with nano-ZnO or nano-Ag induced antimicrobial effects against oral pathogens. Among the nanoparticles, nano-Ag showed the best antimicrobial activity and nano-ZnO showed the highest biocompatibility.</p>","PeriodicalId":51260,"journal":{"name":"Korean Journal of Orthodontics","volume":"53 1","pages":"16-25"},"PeriodicalIF":2.6000,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5b/9a/kjod-53-1-16.PMC9877365.pdf","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Orthodontics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4041/kjod22.091","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 3
Abstract
Objective: We aimed to evaluate the cell viability and antimicrobial effects of orthodontic bands coated with silver or zinc oxide nanoparticles (nano-Ag and nano-ZnO, respectively).
Methods: In this experimental study, 30 orthodontic bands were divided into three groups (n = 10 each): control (uncoated band), Ag (silver-coated band), and ZnO (zinc oxide-coated band). The electrostatic spray-assisted vapor deposition method was used to coat orthodontic bands with nano-Ag or nano-ZnO. The biofilm inhibition test was used to assess the antimicrobial effectiveness of nano-Ag and nano-ZnO against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Biocompatibility tests were conducted using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The groups were compared using oneway analysis of variance with a post-hoc test.
Results: The Ag group showed a significantly higher reduction in the number of L. acidophilus, C. albicans, and S. mutans colonies than the ZnO group (p = 0.015, 0.003, and 0.005, respectively). Compared with the control group, the Ag group showed a 2-log10 reduction in all the microorganisms' replication ability, but only S. mutants showed a 2-log10 reduction in replication ability in the ZnO group. The lowest mean cell viability was observed in the Ag group, but the difference between the groups was insignificant (p > 0.05).
Conclusions: Coating orthodontic bands with nano-ZnO or nano-Ag induced antimicrobial effects against oral pathogens. Among the nanoparticles, nano-Ag showed the best antimicrobial activity and nano-ZnO showed the highest biocompatibility.
期刊介绍：
The Korean Journal of Orthodontics (KJO) is an international, open access, peer reviewed journal published in January, March, May, July, September, and November each year. It was first launched in 1970 and, as the official scientific publication of Korean Association of Orthodontists, KJO aims to publish high quality clinical and scientific original research papers in all areas related to orthodontics and dentofacial orthopedics. Specifically, its interest focuses on evidence-based investigations of contemporary diagnostic procedures and treatment techniques, expanding to significant clinical reports of diverse treatment approaches.
The scope of KJO covers all areas of orthodontics and dentofacial orthopedics including successful diagnostic procedures and treatment planning, growth and development of the face and its clinical implications, appliance designs, biomechanics, TMJ disorders and adult treatment. Specifically, its latest interest focuses on skeletal anchorage devices, orthodontic appliance and biomaterials, 3 dimensional imaging techniques utilized for dentofacial diagnosis and treatment planning, and orthognathic surgery to correct skeletal disharmony in association of orthodontic treatment.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
