In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava.

IF 2.4 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Atla-Alternatives To Laboratory Animals Pub Date : 2023-03-01 DOI:10.1177/02611929231158243

Kalani Gayathri Jayasekara, Preethi Soysa, Thusharie Sugandhika Suresh, Charitha Lakshini Goonasekara, Kamani Mangalika Gunasekera

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Abstract

Dengue is an arboviral (insect-transmitted) infection of global concern. Currently, there are still no specific dengue antiviral agents to treat the disease. Plant extracts have been used in traditional medicine for treating various viral infections - thus, in the present study, aqueous extracts of dried flowers of Aegle marmelos (AM), whole plant of Munronia pinnata (MP) and leaves of Psidium guajava (PG) were investigated for their potential capacity to inhibit dengue virus infection of Vero cells. The maximum non-toxic dose (MNTD) and the 50% cytotoxic concentration (CC₅₀) were determined by using the MTT assay. A plaque reduction antiviral assay was carried out with dengue virus types 1 (DV1), 2 (DV2), 3 (DV3) and 4 (DV4), in order to calculate the half-maximum inhibitory concentration (IC₅₀). AM extract inhibited all four virus serotypes tested; MP extract inhibited DV1, DV2 and DV4, but not DV3; PG extract inhibited DV1, DV2 and DV4, but not DV3. Thus, the results suggest that AM is a promising candidate for the pan-serotype inhibition of dengue viral activity.

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蜜瓜、凤仙花和番石榴水提液对登革病毒的体外抑制作用

登革热是一种全球关注的虫媒病毒(昆虫传播)感染。目前，还没有特异性的登革热抗病毒药物来治疗这种疾病。植物提取物在传统医学中已被用于治疗各种病毒感染，因此，本研究研究了柑橘干花(AM)、芒ronia pinnata (MP)整株和瓜爪哇Psidium guajava (PG)叶的水提取物对登革热病毒感染Vero细胞的潜在抑制能力。采用MTT法测定最大无毒剂量(MNTD)和50%细胞毒浓度(CC50)。采用1型登革热病毒(DV1)、2型登革热病毒(DV2)、3型登革热病毒(DV3)和4型登革热病毒(DV4)进行斑块减少抗病毒实验，计算最大半数抑制浓度(IC50)。AM提取物对四种病毒血清型均有抑制作用;MP提取物对DV1、DV2、DV4有抑制作用，对DV3无抑制作用;PG提取物对DV1、DV2、DV4有抑制作用，对DV3无抑制作用。因此，结果表明AM是一种有希望的泛血清型登革热病毒活性抑制候选物。

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来源期刊

Atla-Alternatives To Laboratory Animals 农林科学-兽医学

CiteScore

3.80

自引率

3.70%

发文量

审稿时长

>18 weeks

期刊介绍： Alternatives to Laboratory Animals (ATLA) is a peer-reviewed journal, intended to cover all aspects of the development, validation, implementation and use of alternatives to laboratory animals in biomedical research and toxicity testing. In addition to the replacement of animals, it also covers work that aims to reduce the number of animals used and refine the in vivo experiments that are still carried out.