Functional improvements in β-conglycinin by edible bioconjugation with carboxymethyl dextran.

IF 1.7 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Cytotechnology Pub Date : 2023-04-01 Epub Date: 2022-12-15 DOI:10.1007/s10616-022-00565-9

Tadashi Yoshida, Misato Tomono, Ryohei Takahashi, Makoto Hattori

引用次数: 0

Abstract

β-Conglycinin was conjugated with carboxymethyl dextran (CMD) by the Maillard reaction to improve its function. The β-conglycinin-CMD conjugate was purified by dialysis. Conjugation was confirmed by SDS-PAGE with CBB and PAS staining. Composition of the β-conglycinin-CMD was β-conglycinin:CMD = 1:2.7 (molar ratio) which was confirmed by BCA method and phenol sulfuric acid method. Solubility of β-conglycinin in the range of pH 2.0-7.0 was much improved by conjugation with CMD. Emulsifying property of β-conglycinin at pH 7 and in presence of salt was improved by conjugation with CMD. Immunogenicity of β-conglycinin was reduced by conjugation with CMD. Conjugation method performed in this study was considered to be valuable in that it can be used in food processing.

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通过与羧甲基葡聚糖进行可食用生物共轭，改善β-红霉素的功能。

通过 Maillard 反应，β-红霉素与羧甲基葡聚糖（CMD）共轭，以改善其功能。β-红霉素-CMD 共轭物经透析纯化。通过 SDS-PAGE 与 CBB 和 PAS 染色确认了共轭作用。经 BCA 法和苯酚硫酸法确认，β-共赖氨酸-CMD 的组成为 β-共赖氨酸：CMD = 1:2.7（摩尔比）。与 CMD 共轭后，β-红景天素在 pH 2.0-7.0 范围内的溶解度大大提高。在 pH 值为 7 且有盐存在的情况下，与 CMD 共轭可改善 β-丛赖霉素的乳化性能。与 CMD 共轭可降低 β-共赖氨酸的免疫原性。本研究中采用的共轭方法可用于食品加工，因此具有重要价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.