Functional improvements in β-conglycinin by preparing edible bioconjugates with ε-polylysine and dextran.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-04-01 Epub Date: 2023-01-05 DOI:10.1007/s10616-022-00568-6

Tadashi Yoshida, Ikumi Hamaji, Takeshi Hashimoto, Takuya Matsumoto, Makoto Hattori

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Abstract

β-Conglycinin was conjugated with ε-polylysine (PL) by means of microbial transglutaminase (MTGase) to improve its function. The β-conglycinin-PL conjugate was purified by dialysis. Composition of the β-conglycinin-PL was β-conglycinin:PL = 1:18 (molar ratio) which was confirmed by amino acid analysis. The β-conglycinin-PL was further conjugated with dextran (Dex) by the Maillard reaction. The β-conglycinin-PL-Dex conjugate was purified by dialysis. Conjugation was confirmed by SDS-PAGE and PAS staining. Composition of the β-conglycinin-PL-Dex was β-conglycinin-PL:Dex = 1:41 (molar ratio) which was confirmed by UV spectra measurement and phenol sulfuric acid method. Solubility of β-conglycinin in the acidic range was much improved by conjugation with PL and further improved by further conjugation with Dex. Emulsifying property of β-conglycinin in acidic pH range was much improved by conjugation with PL and Dex. Immunogenicity of β-conglycinin was decreased by conjugation with PL and Dex.

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用ε-聚赖氨酸和葡聚糖制备可食用生物共轭物，改善β-红霉素的功能。

通过微生物转谷氨酰胺酶（MTGase）将β-红霉素与ε-聚赖氨酸（PL）共轭，以改善其功能。β-红景天素-PL共轭物经透析纯化。经氨基酸分析确认，β-共线磷脂-PL 的组成为 β-共线磷脂：PL = 1:18（摩尔比）。通过 Maillard 反应，β-conglycinin-PL 与右旋糖酐（Dex）进一步共轭。β-红霉素-PL-Dex共轭物经透析纯化。通过 SDS-PAGE 和 PAS 染色确认了共轭作用。经紫外光谱测量和酚硫酸法确认，β-红景天素-PL-地塞米松的组成为β-红景天素-PL:地塞米松=1:41（摩尔比）。与 PL 共轭后，β-红霉素在酸性范围内的溶解度大大提高，与 Dex 进一步共轭后，溶解度进一步提高。与 PL 和 Dex 共轭后，β-粗壮霉素在酸性 pH 范围内的乳化性能大大改善。与 PL 和 Dex 共轭后，β-红霉素的免疫原性降低。

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