Functional improvements in β-conglycinin by preparing edible bioconjugates with ε-polylysine and dextran.

IF 1.7 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Cytotechnology Pub Date : 2023-04-01 Epub Date: 2023-01-05 DOI:10.1007/s10616-022-00568-6

Tadashi Yoshida, Ikumi Hamaji, Takeshi Hashimoto, Takuya Matsumoto, Makoto Hattori

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Abstract

β-Conglycinin was conjugated with ε-polylysine (PL) by means of microbial transglutaminase (MTGase) to improve its function. The β-conglycinin-PL conjugate was purified by dialysis. Composition of the β-conglycinin-PL was β-conglycinin:PL = 1:18 (molar ratio) which was confirmed by amino acid analysis. The β-conglycinin-PL was further conjugated with dextran (Dex) by the Maillard reaction. The β-conglycinin-PL-Dex conjugate was purified by dialysis. Conjugation was confirmed by SDS-PAGE and PAS staining. Composition of the β-conglycinin-PL-Dex was β-conglycinin-PL:Dex = 1:41 (molar ratio) which was confirmed by UV spectra measurement and phenol sulfuric acid method. Solubility of β-conglycinin in the acidic range was much improved by conjugation with PL and further improved by further conjugation with Dex. Emulsifying property of β-conglycinin in acidic pH range was much improved by conjugation with PL and Dex. Immunogenicity of β-conglycinin was decreased by conjugation with PL and Dex.

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用ε-聚赖氨酸和葡聚糖制备可食用生物共轭物，改善β-红霉素的功能。

通过微生物转谷氨酰胺酶（MTGase）将β-红霉素与ε-聚赖氨酸（PL）共轭，以改善其功能。β-红景天素-PL共轭物经透析纯化。经氨基酸分析确认，β-共线磷脂-PL 的组成为 β-共线磷脂：PL = 1:18（摩尔比）。通过 Maillard 反应，β-conglycinin-PL 与右旋糖酐（Dex）进一步共轭。β-红霉素-PL-Dex共轭物经透析纯化。通过 SDS-PAGE 和 PAS 染色确认了共轭作用。经紫外光谱测量和酚硫酸法确认，β-红景天素-PL-地塞米松的组成为β-红景天素-PL:地塞米松=1:41（摩尔比）。与 PL 共轭后，β-红霉素在酸性范围内的溶解度大大提高，与 Dex 进一步共轭后，溶解度进一步提高。与 PL 和 Dex 共轭后，β-粗壮霉素在酸性 pH 范围内的乳化性能大大改善。与 PL 和 Dex 共轭后，β-红霉素的免疫原性降低。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.