Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture of Clostridium beijerinckii G117 and recombinant Bacillus subtilis 1A1

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2020-12-01 DOI:10.1016/j.mec.2020.e00137
Yonghao Cui , Jianzhong He , Kun-Lin Yang , Kang Zhou
{"title":"Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture of Clostridium beijerinckii G117 and recombinant Bacillus subtilis 1A1","authors":"Yonghao Cui ,&nbsp;Jianzhong He ,&nbsp;Kun-Lin Yang ,&nbsp;Kang Zhou","doi":"10.1016/j.mec.2020.e00137","DOIUrl":null,"url":null,"abstract":"<div><p>An engineered <em>B. subtilis</em> 1A1 strain (BsADH2) expressing a secondary alcohol dehydrogenase (CpSADH) was co-cultured with <em>C. beijerinckii</em> G117 under an aerobic condition. During the fermentation on glucose, <em>B. subtilis</em> BsADH2 depleted oxygen in culture media completely and created an anaerobic environment for <em>C. beijerinckii</em> G117, an obligate anaerobe, to grow. Meanwhile, lactate produced by <em>B. subtilis</em> BsADH2 was re-assimilated by <em>C. beijerinckii</em> G117. In return, acetone produced by <em>C. beijerinckii</em> G117 was reduced into isopropanol by <em>B. subtilis</em> BsADH2 via expressing the CpSADH, which helped maintain the redox balance of the engineered <em>B. subtilis</em>. In the symbiotic system consisting of two strains, 1.7 ​g/L of acetone, 4.8 ​g/L of butanol, and 0.9 ​g/L of isopropanol (with an isopropanol/acetone ratio of 0.53) was produced from 60 ​g/L of glucose. This symbiotic system also worked when oxygen was supplied to the culture, although less isopropanol was produced (0.9 ​g/L of acetone, 4.9 ​g/L of butanol, and 0.2 ​g/L of isopropanol). The isopropanol titer was increased substantially to 2.5 ​g/L when we increased the inoculum size of <em>B. subtilis</em> BsADH2 and optimized other process parameters. With the <em>Bacillus</em>-<em>Clostridium</em> co-culture, switching from the original acetone-butanol (AB) fermentation to an aerobic acetone-butanol-isopropanol (ABI) fermentation can be easily achieved without genetic engineering of <em>Clostridium</em>. This strategy of employing a recombinant <em>Bacillus</em> to co-culture with <em>Clostridium</em> should be potentially useful to modify traditional acetone-butanol-ethanol fermentation for the production of other value-added chemicals.</p></div>","PeriodicalId":18695,"journal":{"name":"Metabolic Engineering Communications","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mec.2020.e00137","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolic Engineering Communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214030120300079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 12

Abstract

An engineered B. subtilis 1A1 strain (BsADH2) expressing a secondary alcohol dehydrogenase (CpSADH) was co-cultured with C. beijerinckii G117 under an aerobic condition. During the fermentation on glucose, B. subtilis BsADH2 depleted oxygen in culture media completely and created an anaerobic environment for C. beijerinckii G117, an obligate anaerobe, to grow. Meanwhile, lactate produced by B. subtilis BsADH2 was re-assimilated by C. beijerinckii G117. In return, acetone produced by C. beijerinckii G117 was reduced into isopropanol by B. subtilis BsADH2 via expressing the CpSADH, which helped maintain the redox balance of the engineered B. subtilis. In the symbiotic system consisting of two strains, 1.7 ​g/L of acetone, 4.8 ​g/L of butanol, and 0.9 ​g/L of isopropanol (with an isopropanol/acetone ratio of 0.53) was produced from 60 ​g/L of glucose. This symbiotic system also worked when oxygen was supplied to the culture, although less isopropanol was produced (0.9 ​g/L of acetone, 4.9 ​g/L of butanol, and 0.2 ​g/L of isopropanol). The isopropanol titer was increased substantially to 2.5 ​g/L when we increased the inoculum size of B. subtilis BsADH2 and optimized other process parameters. With the Bacillus-Clostridium co-culture, switching from the original acetone-butanol (AB) fermentation to an aerobic acetone-butanol-isopropanol (ABI) fermentation can be easily achieved without genetic engineering of Clostridium. This strategy of employing a recombinant Bacillus to co-culture with Clostridium should be potentially useful to modify traditional acetone-butanol-ethanol fermentation for the production of other value-added chemicals.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
贝氏梭菌G117与重组枯草芽孢杆菌1A1共培养的好氧丙酮-丁醇-异丙醇(ABI)发酵
在好氧条件下,将表达二醇脱氢酶(CpSADH)的枯草芽孢杆菌1A1 (BsADH2)与beijerinckii C. G117共培养。在葡萄糖发酵过程中,枯草芽孢杆菌BsADH2完全耗尽培养基中的氧气,为专性厌氧菌C. beijerinckii G117的生长创造了厌氧环境。同时,枯草芽孢杆菌BsADH2产生的乳酸被贝氏弧菌G117重新同化。反过来,C. beijerinckii G117产生的丙酮通过表达CpSADH被枯草芽孢杆菌BsADH2还原为异丙醇,这有助于维持工程枯草芽孢杆菌的氧化还原平衡。在由两菌株组成的共生体系中,从60 g/L葡萄糖中产生1.7 g/L丙酮、4.8 g/L丁醇和0.9 g/L异丙醇(异丙醇/丙酮比为0.53)。尽管异丙醇产量较低(丙酮0.9 g/L,丁醇4.9 g/L,异丙醇0.2 g/L),但向培养物提供氧气时,这种共生系统也起作用。通过增加枯草芽孢杆菌BsADH2的接种量和优化其他工艺参数,使异丙醇滴度大幅提高至2.5 g/L。通过芽孢杆菌与梭状芽孢杆菌的共培养,可以在不需要梭状芽孢杆菌基因工程的情况下,很容易地实现由原来的丙酮-丁醇(AB)发酵向需氧丙酮-丁醇-异丙醇(ABI)发酵的转变。这种利用重组芽孢杆菌与梭状芽孢杆菌共培养的策略可能有助于改进传统的丙酮-丁醇-乙醇发酵,以生产其他增值化学品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
期刊最新文献
Metabolic engineering of Acinetobacter baylyi ADP1 for naringenin production PEZy-miner: An artificial intelligence driven approach for the discovery of plastic-degrading enzyme candidates Production of (R)-citramalate by engineered Saccharomyces cerevisiae Engineering thioesterase as a driving force for novel itaconate production via its degradation scheme A comparative analysis of NADPH supply strategies in Saccharomyces cerevisiae: Production of d-xylitol from d-xylose as a case study
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1