Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture of Clostridium beijerinckii G117 and recombinant Bacillus subtilis 1A1

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2020-12-01 DOI:10.1016/j.mec.2020.e00137
Yonghao Cui , Jianzhong He , Kun-Lin Yang , Kang Zhou
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引用次数: 12

Abstract

An engineered B. subtilis 1A1 strain (BsADH2) expressing a secondary alcohol dehydrogenase (CpSADH) was co-cultured with C. beijerinckii G117 under an aerobic condition. During the fermentation on glucose, B. subtilis BsADH2 depleted oxygen in culture media completely and created an anaerobic environment for C. beijerinckii G117, an obligate anaerobe, to grow. Meanwhile, lactate produced by B. subtilis BsADH2 was re-assimilated by C. beijerinckii G117. In return, acetone produced by C. beijerinckii G117 was reduced into isopropanol by B. subtilis BsADH2 via expressing the CpSADH, which helped maintain the redox balance of the engineered B. subtilis. In the symbiotic system consisting of two strains, 1.7 ​g/L of acetone, 4.8 ​g/L of butanol, and 0.9 ​g/L of isopropanol (with an isopropanol/acetone ratio of 0.53) was produced from 60 ​g/L of glucose. This symbiotic system also worked when oxygen was supplied to the culture, although less isopropanol was produced (0.9 ​g/L of acetone, 4.9 ​g/L of butanol, and 0.2 ​g/L of isopropanol). The isopropanol titer was increased substantially to 2.5 ​g/L when we increased the inoculum size of B. subtilis BsADH2 and optimized other process parameters. With the Bacillus-Clostridium co-culture, switching from the original acetone-butanol (AB) fermentation to an aerobic acetone-butanol-isopropanol (ABI) fermentation can be easily achieved without genetic engineering of Clostridium. This strategy of employing a recombinant Bacillus to co-culture with Clostridium should be potentially useful to modify traditional acetone-butanol-ethanol fermentation for the production of other value-added chemicals.

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贝氏梭菌G117与重组枯草芽孢杆菌1A1共培养的好氧丙酮-丁醇-异丙醇(ABI)发酵
在好氧条件下,将表达二醇脱氢酶(CpSADH)的枯草芽孢杆菌1A1 (BsADH2)与beijerinckii C. G117共培养。在葡萄糖发酵过程中,枯草芽孢杆菌BsADH2完全耗尽培养基中的氧气,为专性厌氧菌C. beijerinckii G117的生长创造了厌氧环境。同时,枯草芽孢杆菌BsADH2产生的乳酸被贝氏弧菌G117重新同化。反过来,C. beijerinckii G117产生的丙酮通过表达CpSADH被枯草芽孢杆菌BsADH2还原为异丙醇,这有助于维持工程枯草芽孢杆菌的氧化还原平衡。在由两菌株组成的共生体系中,从60 g/L葡萄糖中产生1.7 g/L丙酮、4.8 g/L丁醇和0.9 g/L异丙醇(异丙醇/丙酮比为0.53)。尽管异丙醇产量较低(丙酮0.9 g/L,丁醇4.9 g/L,异丙醇0.2 g/L),但向培养物提供氧气时,这种共生系统也起作用。通过增加枯草芽孢杆菌BsADH2的接种量和优化其他工艺参数,使异丙醇滴度大幅提高至2.5 g/L。通过芽孢杆菌与梭状芽孢杆菌的共培养,可以在不需要梭状芽孢杆菌基因工程的情况下,很容易地实现由原来的丙酮-丁醇(AB)发酵向需氧丙酮-丁醇-异丙醇(ABI)发酵的转变。这种利用重组芽孢杆菌与梭状芽孢杆菌共培养的策略可能有助于改进传统的丙酮-丁醇-乙醇发酵,以生产其他增值化学品。
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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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