MiRNA-200b level in peripheral blood predicts renal interstitial injury in patients with diabetic nephropathy.

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Medical Biochemistry Pub Date : 2023-03-15 DOI:10.5937/jomb0-40379

Tingfang Chen, Zhenzhen Jiang, Haiying Zhang, Ruifeng Yang, Yan Wu, Yongping Guo

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Abstract

Background: To uncover the diagnostic potential of peripheral blood microRNA-200b (miRNA-200b) in renal interstitial injury in diabetic nephropathy (DN) patients.

Methods: A total of 50 diabetes subjects, 50 mild DN subjects, 50 moderate-severe DN subjects and 50 healthy subjects were included. Peripheral blood level of miRNA-200b in every subject was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Serum levels of renal function indicators were determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, relative levels of fibrosis damage indicators were examined by chemiluminescent immunoassay. Diagnostic potentials of miRNA200b in diabetes, mild DN and moderate-severe DN were assessed by depicting receiver operating characteristic (ROC) curves.

Results: Peripheral blood level of miRNA-200b was higher in DN subjects than diabetes subjects without vascular complications, especially moderate-severe DN patients. Peripheral blood level of miRNA-200b in DN subjects was negatively correlated to relative levels of serum creatinine, urinary nitrogen, cystatin, TGF-b, CIV and PCIII. ROC curves demonstrated diagnostic potentials of miRNA-200b in mild and moderate-severe DN.

Conclusions: Peripheral blood level of miRNA-200b is closely linked to the degree of renal interstitial injury in DN patients. MiRNA-200b may be a vital indicator in predicting the development of DN.

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外周血MiRNA-200b水平可预测糖尿病肾病患者肾间质损伤。

背景:揭示外周血microRNA-200b (miRNA-200b)在糖尿病肾病(DN)患者肾间质损伤中的诊断潜力。方法:糖尿病患者50例，轻度DN患者50例，中重度DN患者50例，健康者50例。采用逆转录聚合酶链反应(RT-PCR)检测各组患者外周血miRNA-200b水平。采用酶联免疫吸附试验(ELISA)测定血清肾功能指标水平。同时，采用化学发光免疫分析法检测纤维化损伤指标的相对水平。通过绘制受试者工作特征(ROC)曲线评估miRNA200b在糖尿病、轻度DN和中重度DN中的诊断潜力。结果:DN患者外周血miRNA-200b水平高于无血管并发症的糖尿病患者，尤其是中重度DN患者。DN患者外周血miRNA-200b水平与血清肌酐、尿氮、胱抑素、TGF-b、CIV、PCIII相对水平呈负相关。ROC曲线显示miRNA-200b在轻度和中重度DN中的诊断潜力。结论:外周血miRNA-200b水平与DN患者肾间质损伤程度密切相关。MiRNA-200b可能是预测DN发展的重要指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.