Optimization of quorum quenching mediated bacterial attenuation of Solanum torvum root extract by response surface modelling through Box-Behnken approach

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI:10.1016/j.jgeb.2018.02.001

Kayeen Vadakkan , Selvaraj Vijayanand , Abbas Alam Choudhury , Ramya Gunasekaran , Janarthanam Hemapriya

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引用次数: 8

Abstract

The present study was intended to optimize the quorum sensing inhibitory action of Solanum torvum root extract against Chromobacterium violaceum. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for optimizing quorum quenching activity of extract. The adequacy of mathematical model was verified by ANOVA and Cook’s distance table. Results revealed that quorum quenching property of Solanum torvum root extract is highly influenced by variables studied whereas maximum activity was found during administration of 300 µg/ml extract thrice in a day. It was also understood that extract does not possess any bactericidal activity wherein it only silence its quorum sensing mediated functions. This observations can be further used in quorum quenching studies.

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基于Box-Behnken法的响应面建模优化群体淬灭介导的托鲁巴根提取物细菌衰减

本研究旨在优化torvum根提取物对紫色杆菌的群体感应抑制作用。分析了细菌密度、给药频率和提取物浓度等因素。植物样本采集自印度喀拉拉邦Thrissur地区。采用Box-Behnken法对各因素进行响应面建模，优化提取物的群体猝灭活性。通过方差分析和库克距离表验证数学模型的充分性。结果表明，torvum根提取物的群体猝灭特性受研究变量的影响较大，其中300 µg/ml提取物每日3次时活性最大。还了解到，提取物不具有任何杀菌活性，其中它仅沉默其群体感应介导的功能。这一观察结果可以进一步用于群体猝灭研究。

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts