{"title":"Biosynthesis of riboflavin","authors":"Peter Nielsen, Adelbert Bacher","doi":"10.1016/0005-2744(81)90044-9","DOIUrl":null,"url":null,"abstract":"<div><p>The 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5′-phosphate deaminase was partially purified from cell extracts of <em>Candida guilliermondii</em> ATCC 9058. The enzyme requires Mg<sup>2+</sup> for activity. Maximal activity was observed at pH 7.3. The enzyme converts its substrate, 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5′-phosphate, to 2,5-diamino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5′-phosphate. This labile compound was treated with diacetyl and the resulting 6,7-dimethyl-8-ribityllumazine 5′-phosphate was identified by comparison with a synthetic sample.</p></div>","PeriodicalId":100159,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology","volume":"662 2","pages":"Pages 312-317"},"PeriodicalIF":0.0000,"publicationDate":"1981-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2744(81)90044-9","citationCount":"38","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005274481900449","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 38
Abstract
The 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5′-phosphate deaminase was partially purified from cell extracts of Candida guilliermondii ATCC 9058. The enzyme requires Mg2+ for activity. Maximal activity was observed at pH 7.3. The enzyme converts its substrate, 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5′-phosphate, to 2,5-diamino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5′-phosphate. This labile compound was treated with diacetyl and the resulting 6,7-dimethyl-8-ribityllumazine 5′-phosphate was identified by comparison with a synthetic sample.
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