Polymorphisms in ERCC1, ERCC4 and ERCC5 genes as biomarkers of susceptibility for pesticide-induced DNA damage in North-West Indian agricultural workers.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biomarkers Pub Date : 2023-12-01 Epub Date: 2023-12-11 DOI:10.1080/1354750X.2023.2284109

Karashdeep Kaur, Rupinder Kaur

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Abstract

Background: Occupational pesticides exposure has raised health concerns due to genotoxicity and accumulation of DNA damage. Polymorphisms in genes encoding enzymes involved in nucleotide excision repair (NER) may affect the individual's susceptibility to pesticide toxicity.

Methods: This study evaluates the association of excision repair cross complementation group 1 (ERCC1) (8092 C > A, 3'UTR, rs3212986) and ERCC1 (19007 C > T, Asn118Asn, rs11615), ERCC4 (1244 G > A, Arg415Gln, rs1800067) and ERCC5 (3507 G > C, Asp1104His, rs17655) polymorphisms with pesticide-induced DNA damage in North-West Indian agricultural workers. The study population comprised 225 agricultural workers exposed to pesticides and 225 non-exposed controls.

Results: Our study demonstrate that exposed workers carrying variant ERCC1 8092AA genotype showed higher total comet DNA migration (p = 0.015) as well as increased frequency of cells showing DNA migration (p = 0.027). Exposed agricultural workers with variant ERCC4 1244AA (415Gln/Gln) and ERCC5 3507CC (1104His/His) genotypes exhibited elevation in total comet DNA migration (p < 0.01). However, genotypes of ERCC1 19007 C > T (Asn118Asn) showed no association with total comet DNA migration (p = 0.963), frequency of cells showing DNA migration (p = 0.423) as well as mean tail length (p = 0.432).

Conclusion: ERCC1, ERCC4 and ERCC5 polymorphisms influence DNA damage and can be used as biomarkers of susceptibility for pesticide-induced DNA damage in North-West Indian agricultural workers.

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ERCC1、ERCC4和ERCC5基因多态性作为西北印度农业工人农药诱导DNA损伤易感性的生物标志物

背景:职业性农药暴露由于遗传毒性和DNA损伤积累引起了健康问题。编码核苷酸切除修复(NER)相关酶的基因多态性可能影响个体对农药毒性的易感性。方法:本研究评估了印度西北部农业工人切除修复交叉互补组1 (ERCC1) (8092C > A, 3'UTR, rs3212986)和ERCC1 (19007C > T, Asn118Asn, rs11615)， ERCC4 (1244G > A, Arg415Gln, rs1800067)和ERCC5 (3507G > C, Asp1104His, rs17655)多态性与农药诱导的DNA损伤的关系。研究人群包括225名接触杀虫剂的农业工人和225名未接触杀虫剂的对照者。结果:携带变异ERCC1 8092AA基因型的暴露工人显示出更高的彗星DNA迁移总量(p = 0.015)，出现DNA迁移的细胞频率增加(p = 0.027)。携带变异ERCC4 1244AA (415Gln/Gln)和ERCC5 3507CC (1104His/His)基因型的农业工人暴露在彗星DNA总迁移(p = 0.963)、出现DNA迁移的细胞频率(p = 0.423)和平均尾长(p = 0.432)的位置上，而ERCC1 19007C > T (Asn118Asn)与彗星DNA总迁移无关(p = 0.963)。结论:ERCC1、ERCC4和ERCC5多态性影响DNA损伤，可作为西北印度农业工人农药致DNA损伤易感性的生物标志物。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.