G2-LIKE CAROTENOID REGULATOR (SlGCR) is a positive regulator of lutein biosynthesis in tomato

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY aBIOTECH Pub Date : 2022-11-29 DOI:10.1007/s42994-022-00088-z
Siyan Ren, Yong Yuan, Hsihua Wang, Yang Zhang
{"title":"G2-LIKE CAROTENOID REGULATOR (SlGCR) is a positive regulator of lutein biosynthesis in tomato","authors":"Siyan Ren,&nbsp;Yong Yuan,&nbsp;Hsihua Wang,&nbsp;Yang Zhang","doi":"10.1007/s42994-022-00088-z","DOIUrl":null,"url":null,"abstract":"<div><p>Lutein is an oxygen-containing carotenoid synthesized in plant chloroplasts and chromoplasts. It plays an indispensable role in promoting plant growth and maintaining eye health in humans. The rate-limiting step of lutein biosynthesis is catalyzed by the lycopene ε-cyclase enzyme (LCYE). Although great progress has been made in the identification of transcription factors involved in the lutein biosynthetic pathway, many systematic molecular mechanisms remain to be elucidated. Here, using co-expression analysis, we identified a gene, <i>G2-LIKE CAROTENOID REGULATOR</i> (<i>SlGCR</i>), encoding a GARP G2-like transcription factor, as the potential regulator of <i>SlLCYE</i> in tomato. Silencing of <i>SlGCR</i> reduced the expression of carotenoid biosynthetic genes and the accumulation of carotenoids in tomato leaves. By contrast, overexpression of <i>SlGCR</i> in tomato fruit significantly increased the expression of relevant genes and enhanced the accumulation of carotenoids. SlGCR can directly bind to the <i>SlLCYE</i> promoter and activate its expression. In addition, we also discovered that expression of <i>SlGCR</i> was negatively regulated by the master regulator SlRIN, thereby inhibiting lutein synthesis during tomato fruit ripening. Taken together, we identified SlGCR as a novel regulator involved in tomato lutein biosynthesis, elucidated the regulatory mechanism, and provided a potential tool for tomato lutein metabolic engineering.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00088-z.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"aBIOTECH","FirstCategoryId":"1091","ListUrlMain":"https://link.springer.com/article/10.1007/s42994-022-00088-z","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 2

Abstract

Lutein is an oxygen-containing carotenoid synthesized in plant chloroplasts and chromoplasts. It plays an indispensable role in promoting plant growth and maintaining eye health in humans. The rate-limiting step of lutein biosynthesis is catalyzed by the lycopene ε-cyclase enzyme (LCYE). Although great progress has been made in the identification of transcription factors involved in the lutein biosynthetic pathway, many systematic molecular mechanisms remain to be elucidated. Here, using co-expression analysis, we identified a gene, G2-LIKE CAROTENOID REGULATOR (SlGCR), encoding a GARP G2-like transcription factor, as the potential regulator of SlLCYE in tomato. Silencing of SlGCR reduced the expression of carotenoid biosynthetic genes and the accumulation of carotenoids in tomato leaves. By contrast, overexpression of SlGCR in tomato fruit significantly increased the expression of relevant genes and enhanced the accumulation of carotenoids. SlGCR can directly bind to the SlLCYE promoter and activate its expression. In addition, we also discovered that expression of SlGCR was negatively regulated by the master regulator SlRIN, thereby inhibiting lutein synthesis during tomato fruit ripening. Taken together, we identified SlGCR as a novel regulator involved in tomato lutein biosynthesis, elucidated the regulatory mechanism, and provided a potential tool for tomato lutein metabolic engineering.

Abstract Image

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
G2类胡萝卜素调节因子(SlGCR)是番茄叶黄素生物合成的正调节因子
叶黄素是一种在植物叶绿体和色质体中合成的含氧类胡萝卜素。它在促进植物生长和维护人类眼睛健康方面发挥着不可或缺的作用。叶黄素生物合成的限速步骤是由番茄红素ε-环化酶(LCYE)催化的。尽管在识别参与叶黄素生物合成途径的转录因子方面取得了很大进展,但许多系统的分子机制仍有待阐明。在这里,使用共表达分析,我们鉴定了一个编码GARP G2样转录因子的基因,即G2样CAROTENOID调节剂(SlGCR),作为番茄SlLCYE的潜在调节剂。SlGCR的沉默降低了番茄叶片中类胡萝卜素生物合成基因的表达和类胡萝卜素的积累。相反,SlGCR在番茄果实中的过表达显著增加了相关基因的表达,并增强了类胡萝卜素的积累。SlGCR可以直接与SlLCYE启动子结合并激活其表达。此外,我们还发现SlGCR的表达受到主调节因子SlRIN的负调控,从而抑制番茄果实成熟过程中叶黄素的合成。总之,我们确定SlGCR是一种参与番茄叶黄素生物合成的新型调控因子,阐明了其调控机制,并为番茄叶黄素代谢工程提供了一种潜在的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
7.70
自引率
2.80%
发文量
0
期刊最新文献
A simple, highly efficient Agrobacterium tumefaciens‐mediated moss transformation system with broad applications Inference and prioritization of tissue-specific regulons in Arabidopsis and Oryza The acyl-CoA-binding protein VdAcb1 is essential for carbon starvation response and contributes to virulence in Verticillium dahliae Correction: Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development Simultaneous genetic transformation and genome editing of mixed lines in soybean (Glycine max) and maize (Zea mays)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1