The induced mutant allele flo4-303 confers floury characteristics on the japonica rice cultivar 'Hoshinoko'.

IF 2 4区 农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2022-12-01 DOI:10.1270/jsbbs.22059
Shuichi Matsuba, Wakako Maruyama-Funatsuki, Takayuki Umemoto, Hideki Kato, Makoto Kuroki, Narifumi Yokogami, Tomohito Ikegaya, Hiroyuki Shimizu, Norio Iriki
{"title":"The induced mutant allele <i>flo4-303</i> confers floury characteristics on the japonica rice cultivar 'Hoshinoko'.","authors":"Shuichi Matsuba,&nbsp;Wakako Maruyama-Funatsuki,&nbsp;Takayuki Umemoto,&nbsp;Hideki Kato,&nbsp;Makoto Kuroki,&nbsp;Narifumi Yokogami,&nbsp;Tomohito Ikegaya,&nbsp;Hiroyuki Shimizu,&nbsp;Norio Iriki","doi":"10.1270/jsbbs.22059","DOIUrl":null,"url":null,"abstract":"<p><p>Rice flour is useful as a substitute for wheat flour, however, to obtain fine flour, millers need special milling facilities, which increase the cost of milling. To reduce the milling cost, we developed a floury mutant line by irradiating gamma-rays to dry seeds of the japonica cultivar 'Hoshinoyume'. The line was registered as a new cultivar, 'Hoshinoko'. Genetical analysis of the floury trait was conducted using an F<sub>2</sub> population derived from a cross between 'Hoshinoko' and 'Corbetti' (a japonica rice cultivar with normal endosperm), which indicated the involvement of a single recessive gene located near the RM163 marker on the long arm of rice chromosome 5, flanking <i>flo4</i> identified by Kang <i>et al.</i> (2005). Sequence analysis of <i>flo4</i> showed a two-bp (CA) insertion in the eighth exon of in 'Hoshinoko' compared to that of 'Hoshinoyume', which led to a frameshift mutation. The CAPS-based genotype of <i>flo4</i> gene completely correlated to the phenotype of endosperm in two populations. This CAPS marker could be helpful for rice breeders to develop new cultivars harboring floury endosperm of the <i>flo4-303</i> gene.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895802/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breeding Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1270/jsbbs.22059","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 2

Abstract

Rice flour is useful as a substitute for wheat flour, however, to obtain fine flour, millers need special milling facilities, which increase the cost of milling. To reduce the milling cost, we developed a floury mutant line by irradiating gamma-rays to dry seeds of the japonica cultivar 'Hoshinoyume'. The line was registered as a new cultivar, 'Hoshinoko'. Genetical analysis of the floury trait was conducted using an F2 population derived from a cross between 'Hoshinoko' and 'Corbetti' (a japonica rice cultivar with normal endosperm), which indicated the involvement of a single recessive gene located near the RM163 marker on the long arm of rice chromosome 5, flanking flo4 identified by Kang et al. (2005). Sequence analysis of flo4 showed a two-bp (CA) insertion in the eighth exon of in 'Hoshinoko' compared to that of 'Hoshinoyume', which led to a frameshift mutation. The CAPS-based genotype of flo4 gene completely correlated to the phenotype of endosperm in two populations. This CAPS marker could be helpful for rice breeders to develop new cultivars harboring floury endosperm of the flo4-303 gene.

Abstract Image

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
诱变等位基因flo4-303使粳稻品种星野子具有面粉性状。
米粉作为小麦粉的替代品是有用的,然而,为了获得细粉,磨坊需要特殊的碾磨设备,这增加了碾磨的成本。为了降低碾磨成本,我们利用伽玛射线辐照粳稻‘星之玉’的干种子,开发了一种面粉突变系。该品系被注册为新品种“星野子”。利用“星野子”(Hoshinoko)和“Corbetti”(一种具有正常胚乳的粳稻品种)杂交的F2群体对面粉性状进行了遗传分析,结果表明,Kang等人(2005)发现,位于水稻5号染色体长臂RM163标记附近的单隐性基因参与了面粉性状的遗传分析。序列分析显示,与“Hoshinoyume”相比,“Hoshinoko”的第8外显子插入了2 bp (CA),这导致了移码突变。在两个种群中,flo4基因的caps基因型与胚乳表型完全相关。该CAPS标记可为水稻育种者培育含有fl4 -303基因的花粉胚乳新品种提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
期刊最新文献
Detection and validation of QTLs for green stem disorder of soybean (Glycine max (L.) Merr.). Development of genomic and genetic resources facilitating molecular genetic studies on untapped Myanmar rice germplasms. DNAMarkMaker: streamlining ARMS and CAPS marker development from resequencing data with NGS short reads. Interaction between genetic regions responsible for the starch properties in non-glutinous rice varieties in Hokkaido, Japan. NARO historical phenotype dataset from rice breeding.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1