Serum autoantibodies and exploratory molecular pathways in rural miners: A pilot study

IF 4.7 Q2 IMMUNOLOGY Journal of Translational Autoimmunity Pub Date : 2023-01-01 DOI:10.1016/j.jtauto.2023.100197
Esther Erdei , Xixi Zhou , Chris Shuey , Nour Ass'ad , Kimberly Page , Bobbi Gore , Chengsong Zhu , Deborah Kanda , Li Luo , Akshay Sood , Katherine E. Zychowski
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Abstract

Introduction

The Southwestern United States (SWUS) has an extensive history of coal and metal mining, including uranium (U) mining. Lung diseases, including but not limited to, lung cancer and pulmonary fibrosis, have been studied extensively in miners due to occupational, dust-related exposures. However, high-throughput autoimmune biomarkers are largely understudied in miners, despite the fact that ore miners, such as U-miners, are at an increased risk for the development of autoimmune diseases such as systemic sclerosis and systemic lupus erythematosus (SLE). Additionally, there are current gaps in knowledge regarding which signaling pathways may play a role in occupational exposure-associated autoimmunity.

Methods

Most current and former miners in the SWUS live close to their previous workplaces, in remote areas, with limited access to healthcare. In this pilot study, by leveraging a mobile clinical platform for patient care and clinical outreach, we recruited 44 miners who self-identified as either U (n = 10) or non-U miners (n = 34) and received health screenings. Serum IgG and IgM autoantibodies against 128 antigens were assessed using a high-throughput molecular technique, as a preliminary health screening opportunity.

Results

Even when adjusting for age as a covariate, there was a significant (p < 0.05) association between self-reported U-mining exposure and biomarkers including IgM alpha-actinin, histones H2B, and H4, myeloperoxidase (MPO) and myelin basic protein. However, adjusting for age did not result in significant associations for IgG autoantibody production in U-miners. Bioinformatic pathway analysis revealed several altered signaling pathways between IgM and IgG autoantibodies among both U and non-U miners.

Conclusions

Further research is warranted regarding the mechanistic connection between U-exposure and autoantibody development, especially regarding histone-related alterations and IgM autoantibody production.

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农村矿工血清自身抗体和探索性分子途径:一项初步研究。
简介:美国西南部(SWUS)有着丰富的煤炭和金属开采历史,包括铀(U)开采。由于职业性粉尘暴露,包括但不限于肺癌和肺纤维化在内的肺部疾病已在矿工中进行了广泛研究。然而,尽管U-miner等矿工患自身免疫性疾病(如系统性硬化症和系统性红斑狼疮)的风险增加,但矿工体内的高通量自身免疫性生物标志物在很大程度上研究不足。此外,关于哪些信号通路可能在职业暴露相关的自身免疫中发挥作用,目前还存在知识空白。方法:SWUS的大多数现任和前任矿工都住在偏远地区,离他们以前的工作场所很近,获得医疗保健的机会有限。在这项试点研究中,通过利用移动临床平台进行患者护理和临床推广,我们招募了44名自称U(n=10)或非U矿工(n=34)的矿工,并接受了健康筛查。使用高通量分子技术评估针对128种抗原的血清IgG和IgM自身抗体,作为初步健康筛查的机会。结果:即使将年龄作为协变量进行调整,也存在显著的(p结论:需要进一步研究U暴露与自身抗体发展之间的机制联系,特别是组蛋白相关改变和IgM自身抗体产生。
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来源期刊
Journal of Translational Autoimmunity
Journal of Translational Autoimmunity Medicine-Immunology and Allergy
CiteScore
7.80
自引率
2.60%
发文量
33
审稿时长
55 days
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