Noncoding RNA-Associated Competing Endogenous RNA Networks in Doxorubicin-Induced Cardiotoxicity.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY DNA and cell biology Pub Date : 2022-07-01 DOI:10.1089/dna.2022.0022
Zijun Xiao, Shanshan Wei, Jie Huang, Jiaqin Liu, Jian Liu, Bikui Zhang, Wenqun Li
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引用次数: 2

Abstract

Accumulating evidence has indicated that noncoding RNAs (ncRNAs) are involved in doxorubicin-induced cardiotoxicity (DIC). However, the ncRNA-associated competing endogenous RNA (ceRNA)-mediated regulatory mechanisms in DIC remain unclear. In this study, we aimed to systematically investigate the alterations in expression levels of long noncoding RNA (lncRNA), circular RNA (circRNA), microRNA (miRNA), and mRNA in a DIC mouse model through deep RNA sequencing (RNA-seq). The results showed that 217 lncRNAs, 41 circRNAs, 11 miRNAs and 3633 mRNAs were aberrantly expressed. Moreover, the expression of 12 randomly selected transcripts was determined by real-time quantitative polymerase chain reaction to test the reliability of RNA-seq data. Based on the interaction between miRNAs and mRNAs, as well as lncRNAs/circRNAs and miRNAs, we constructed comprehensive lncRNA or circRNA-associated ceRNA networks in DIC mice. Moreover, we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses for differentially expressed genes. In conclusion, these identified ceRNA interactions provide new insight into the underlying mechanism and may be crucial therapeutic targets of DIC.

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非编码RNA相关的竞争内源性RNA网络在阿霉素诱导的心脏毒性。
越来越多的证据表明,非编码rna (ncRNAs)参与了阿霉素诱导的心脏毒性(DIC)。然而,DIC中ncrna相关的竞争内源性RNA (ceRNA)介导的调控机制尚不清楚。在这项研究中,我们旨在通过深度RNA测序(RNA-seq)系统地研究长链非编码RNA (lncRNA)、环状RNA (circRNA)、microRNA (miRNA)和mRNA在DIC小鼠模型中的表达水平变化。结果显示,217个lncrna、41个circrna、11个mirna和3633个mrna异常表达。此外,随机选择12个转录本,通过实时定量聚合酶链反应检测其表达,以检验RNA-seq数据的可靠性。基于mirna与mrna的相互作用,以及lncRNA / circrna与mirna的相互作用,我们在DIC小鼠中构建了完整的lncRNA或circrna相关的ceRNA网络。此外,我们对差异表达基因进行了基因本体和京都基因与基因组百科全书途径富集分析。总之,这些鉴定出的ceRNA相互作用为DIC的潜在机制提供了新的见解,可能是DIC的关键治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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