{"title":"Comparative analyses of <i>Stvb</i>-allelic genes reveal <i>japonica</i> specificity of rice stripe resistance in <i>Oryza sativa</i>.","authors":"Keiko Hayashi, Yoshihiro Kawahara, Hideo Maeda, Yuriko Hayano-Saito","doi":"10.1270/jsbbs.22027","DOIUrl":null,"url":null,"abstract":"<p><p>Rice stripe, a viral disease, causes widespread damage to <i>japonica</i> rice (<i>Oryza sativa</i> ssp. <i>japonica</i>). A rice stripe virus (RSV) bioassay revealed that many <i>indica</i> and <i>japonica</i> upland varieties exhibit resistance, whereas <i>japonica</i> paddy varieties are susceptible. However, the genetic background for this subspecies-dependent resistance is unclear. Herein, we focused on rice stripe resistance genes located at the <i>Stvb</i> locus. Three resistant alleles, <i>Stvb-i</i> (<i>indica</i>), <i>Stvb</i> (<i>japonica</i> upland), and <i>Stvb-o</i> (<i>Oryza officinalis</i>) were compared with the susceptible allele, <i>stvb-j</i> (<i>japonica</i> paddy). The expression of the resistance genes was higher than that of <i>stvb-j</i>. Sequence comparison revealed that the resistant and susceptible alleles had different 5'-end sequences and 61-bp element(s) in the fourth intron. The insertion of an LTR-retrotransposon modified the exon 1 sequence of <i>stvb-j</i>. We then developed four DNA markers based on gene structure information and genotyped resistant and susceptible varieties. The LTR-retrotransposon insertion was detected only in susceptible varieties. Resistant genotypes were primarily found in <i>indica</i> and upland <i>japonica</i>, whereas paddy <i>japonica</i> carried the susceptible genotype. Our results characterize the genetic differences associated with RSV resistance and susceptibility in <i>O. sativa</i> and provide insights on the application of DNA markers in rice stripe disease management.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"333-342"},"PeriodicalIF":2.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895804/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breeding Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1270/jsbbs.22027","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Rice stripe, a viral disease, causes widespread damage to japonica rice (Oryza sativa ssp. japonica). A rice stripe virus (RSV) bioassay revealed that many indica and japonica upland varieties exhibit resistance, whereas japonica paddy varieties are susceptible. However, the genetic background for this subspecies-dependent resistance is unclear. Herein, we focused on rice stripe resistance genes located at the Stvb locus. Three resistant alleles, Stvb-i (indica), Stvb (japonica upland), and Stvb-o (Oryza officinalis) were compared with the susceptible allele, stvb-j (japonica paddy). The expression of the resistance genes was higher than that of stvb-j. Sequence comparison revealed that the resistant and susceptible alleles had different 5'-end sequences and 61-bp element(s) in the fourth intron. The insertion of an LTR-retrotransposon modified the exon 1 sequence of stvb-j. We then developed four DNA markers based on gene structure information and genotyped resistant and susceptible varieties. The LTR-retrotransposon insertion was detected only in susceptible varieties. Resistant genotypes were primarily found in indica and upland japonica, whereas paddy japonica carried the susceptible genotype. Our results characterize the genetic differences associated with RSV resistance and susceptibility in O. sativa and provide insights on the application of DNA markers in rice stripe disease management.
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