Comparative analyses of Stvb-allelic genes reveal japonica specificity of rice stripe resistance in Oryza sativa.

IF 2 4区 农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2022-12-01 DOI:10.1270/jsbbs.22027
Keiko Hayashi, Yoshihiro Kawahara, Hideo Maeda, Yuriko Hayano-Saito
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Abstract

Rice stripe, a viral disease, causes widespread damage to japonica rice (Oryza sativa ssp. japonica). A rice stripe virus (RSV) bioassay revealed that many indica and japonica upland varieties exhibit resistance, whereas japonica paddy varieties are susceptible. However, the genetic background for this subspecies-dependent resistance is unclear. Herein, we focused on rice stripe resistance genes located at the Stvb locus. Three resistant alleles, Stvb-i (indica), Stvb (japonica upland), and Stvb-o (Oryza officinalis) were compared with the susceptible allele, stvb-j (japonica paddy). The expression of the resistance genes was higher than that of stvb-j. Sequence comparison revealed that the resistant and susceptible alleles had different 5'-end sequences and 61-bp element(s) in the fourth intron. The insertion of an LTR-retrotransposon modified the exon 1 sequence of stvb-j. We then developed four DNA markers based on gene structure information and genotyped resistant and susceptible varieties. The LTR-retrotransposon insertion was detected only in susceptible varieties. Resistant genotypes were primarily found in indica and upland japonica, whereas paddy japonica carried the susceptible genotype. Our results characterize the genetic differences associated with RSV resistance and susceptibility in O. sativa and provide insights on the application of DNA markers in rice stripe disease management.

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stvb等位基因的比较分析揭示了水稻条纹抗性的粳稻特异性。
稻条病是一种病毒性病害,对粳稻造成广泛危害。粳稻)。水稻条纹病毒(RSV)生物测定表明,许多籼稻和粳稻旱稻品种表现出抗性,而粳稻水稻品种则表现出易感。然而,这种亚种依赖性抗性的遗传背景尚不清楚。本文主要研究了位于Stvb位点的水稻条纹抗性基因。将3个抗性等位基因Stvb-i(籼稻)、Stvb(旱稻)和Stvb-o(稻)与敏感等位基因Stvb- j(粳稻)进行比较。抗性基因的表达量高于stvb-j。序列比较发现,抗性和易感等位基因的5′端序列和第4内含子的61bp元件不同。ltr -反转录转座子的插入改变了stvb-j的外显子1序列。然后,我们根据基因结构信息和抗性和易感品种的基因分型开发了四种DNA标记。ltr -反转录转座子插入仅在易感品种中检测到。抗性基因型主要在籼稻和旱稻中发现,而水稻粳则携带敏感基因型。我们的研究结果表征了水稻对RSV抗性和易感性的遗传差异,并为DNA标记在水稻条纹病管理中的应用提供了见解。
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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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