Design, alpha testing, and beta testing of a 3-D printed open-hardware portable cryopreservation device for aquatic species.

Abstract

Efforts in development of germplasm repositories to preserve genetic resources of aquatic species are impeded globally by a lack of standardized, inexpensive, reproducible, and portable cryopreservation technologies. The present work demonstrates a 3-D printed standardizable freezing device that can be used with nitrogen vapor shipping dewars for on-site sperm cryopreservation for aquatic species and be distributed as open-source The SDPCD could hold 22 French straws (0.25-mL or 0.5-mL) and a quick-release ring design could eject straws directly into a canister inside a dewar by pressing a button after freezing. The final prototypes produced cooling rates of 1 to 64 °C/min for 0.25-mL straws, and 3 to 37 °C/min for 0.5-mL straws with material cost of US$3.5 for a single device and US$1,820-2,562 for batch production of 20 replicates. Progressing through design, prototyping, and testing was delineated to help guide development of other open-source devices within cryopreservation user communities.