Jenna Kropp Schmidt, Matthew R Reynolds, Thaddeus G Golos, Igor I Slukvin
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引用次数: 0
Abstract
Nonhuman primates (NHPs) are well-established basic and translational research models for human immunodeficiency virus (HIV) infections and pathophysiology, hematopoietic stem cell (HSC) transplantation, and assisted reproductive technologies. Recent advances in CRISPR/Cas9 gene editing technologies present opportunities to refine NHP HIV models for investigating genetic factors that affect HIV replication and designing cellular therapies that exploit genetic barriers to HIV infections, including engineering mutations into CCR5 and conferring resistance to HIV/simian immunodeficiency virus (SIV) infections. In this report, we provide an overview of recent advances and challenges in gene editing NHP embryos and discuss the value of genetically engineered animal models for developing novel stem cell-based therapies for curing HIV.
非人类灵长类动物（NHPs）是人类免疫缺陷病毒（HIV）感染和病理生理学、造血干细胞（HSC）移植以及辅助生殖技术的成熟基础研究和转化研究模型。CRISPR/Cas9 基因编辑技术的最新进展为完善 NHP HIV 模型提供了机会，可用于研究影响 HIV 复制的遗传因素和设计细胞疗法，利用基因屏障阻止 HIV 感染，包括对 CCR5 进行工程突变和赋予 HIV/西安免疫缺陷病毒（SIV）感染的抵抗力。在本报告中，我们概述了基因编辑 NHP 胚胎的最新进展和挑战，并讨论了基因工程动物模型在开发基于干细胞的新型疗法以治疗 HIV 方面的价值。
期刊介绍：
Retrovirology is an open access, online journal that publishes stringently peer-reviewed, high-impact articles on host-pathogen interactions, fundamental mechanisms of replication, immune defenses, animal models, and clinical science relating to retroviruses. Retroviruses are pleiotropically found in animals. Well-described examples include avian, murine and primate retroviruses.
Two human retroviruses are especially important pathogens. These are the human immunodeficiency virus, HIV, and the human T-cell leukemia virus, HTLV. HIV causes AIDS while HTLV-1 is the etiological agent for adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. Retrovirology aims to cover comprehensively all aspects of human and animal retrovirus research.
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