L-Ascorbic acid and phosphatidylcholine complex vesicles: formation and elucidation of their biological activities, and their molecular interactions.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED Journal of microencapsulation Pub Date : 2023-01-01 DOI:10.1080/02652048.2022.2160845

Thapakorn Tree-Udom, Chalermrat Simavong, Prapasiri Phetklung, Kanjanaporn Chompoonuch, Sagaw Prateepchinda, Supatchaya Jaemsai, Andrew William King, Oraphan King

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Abstract

The aim is to prepare, characterise, and evaluate the biological activities and key molecular interactions of L-ascorbic acid and phosphatidylcholine (PC-AA) complex vesicles. PC-AA complexes were prepared and characterised using DLS, TEM, FTIR, UV-Vis, in-vitro release, bioactivities, and cytotoxicity. The key interactions of the AA with the PC were studied with MD simulations. PC-AA complex provides improved stability towards the degradation of AA in aqueous solutions while also slowing its release profile. The PC-AA complexes with an optimal molar ratio of PC: AA = 2.5:1 was shown to have a hydrodynamic diameter of 368.67 ± 4.65 nm and an EE of 68.16 ± 0.23%. At low concentration, the PC-AA complexes have no toxicity towards human dermal fibroblast cells over 48 h. Importantly, MD suggests that AA only forms the PC-AA complex when in its neutral form which is the desired active form. PC-AA complex could be a potential active to use in medicinal and cosmeceutical applications.

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l -抗坏血酸和磷脂酰胆碱复合物囊泡:其生物活性的形成和阐明及其分子相互作用。

目的是制备、表征和评价l -抗坏血酸和磷脂酰胆碱(PC-AA)复合物囊泡的生物活性和关键的分子相互作用。制备了PC-AA配合物，并用DLS、TEM、FTIR、UV-Vis、体外释放度、生物活性和细胞毒性对其进行了表征。通过MD模拟研究了AA与PC的关键相互作用。PC-AA配合物提高了AA在水溶液中降解的稳定性，同时也减缓了其释放。以PC: AA = 2.5:1为最佳摩尔比的PC-AA配合物的水动力直径为368.67±4.65 nm, EE为68.16±0.23%。在低浓度下，PC-AA复合物对人真皮成纤维细胞在48小时内无毒性。重要的是，MD表明AA仅在中性形式下形成PC-AA复合物，而中性形式是期望的活性形式。PC-AA配合物在医药和药妆领域具有潜在的应用价值。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.