A protein microarray-based serum proteomic investigation reveals distinct autoantibody signature in colorectal cancer.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-03-01 DOI:10.1002/prca.202200062

Abhilash Barpanda, Chaitanya Tuckley, Arka Ray, Arghya Banerjee, Siddhartha P Duttagupta, Chetan Kantharia, Sanjeeva Srivastava

{"title":"A protein microarray-based serum proteomic investigation reveals distinct autoantibody signature in colorectal cancer.","authors":"Abhilash Barpanda, Chaitanya Tuckley, Arka Ray, Arghya Banerjee, Siddhartha P Duttagupta, Chetan Kantharia, Sanjeeva Srivastava","doi":"10.1002/prca.202200062","DOIUrl":null,"url":null,"abstract":"Purpose: Colorectal cancer (CRC) has been reported as the second leading cause of cancer death worldwide. The 5-year annual survival is around 50%, mainly due to late diagnosis, striking necessity for early detection. This study aims to identify autoantibody in patients' sera for early screening of cancer.Experimental design: The study used a high-density human proteome array with approximately 17,000 recombinant proteins. Screening of sera from healthy individuals, CRC from Indian origin, and CRC from middle-east Asia origin were performed. Bio-statistical analysis was performed to identify significant autoantibodies altered. Pathway analysis was performed to explore the underlying mechanism of the disease.Results: The comprehensive proteomic analysis revealed dysregulation of 15 panels of proteins including CORO7, KCNAB1, WRAP53, NDUFS6, KRT30, and COLGALT2. Further biological pathway analysis for the top dysregulated autoantigenic proteins revealed perturbation in important biological pathways such as ECM degradation and cytoskeletal remodeling etc. CONCLUSIONS AND CLINICAL RELEVANCE: The generation of an autoimmune response against cancer-linked pathways could be linked to the screening of the disease. The process of immune surveillance can be detected at an early stage of cancer. Moreover, AAbs can be easily extracted from blood serum through the least invasive test for disease screening.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/prca.202200062","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 5

Abstract

Purpose: Colorectal cancer (CRC) has been reported as the second leading cause of cancer death worldwide. The 5-year annual survival is around 50%, mainly due to late diagnosis, striking necessity for early detection. This study aims to identify autoantibody in patients' sera for early screening of cancer.

Experimental design: The study used a high-density human proteome array with approximately 17,000 recombinant proteins. Screening of sera from healthy individuals, CRC from Indian origin, and CRC from middle-east Asia origin were performed. Bio-statistical analysis was performed to identify significant autoantibodies altered. Pathway analysis was performed to explore the underlying mechanism of the disease.

Results: The comprehensive proteomic analysis revealed dysregulation of 15 panels of proteins including CORO7, KCNAB1, WRAP53, NDUFS6, KRT30, and COLGALT2. Further biological pathway analysis for the top dysregulated autoantigenic proteins revealed perturbation in important biological pathways such as ECM degradation and cytoskeletal remodeling etc. CONCLUSIONS AND CLINICAL RELEVANCE: The generation of an autoimmune response against cancer-linked pathways could be linked to the screening of the disease. The process of immune surveillance can be detected at an early stage of cancer. Moreover, AAbs can be easily extracted from blood serum through the least invasive test for disease screening.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

一项基于蛋白质微阵列的血清蛋白质组学研究揭示了结直肠癌中独特的自身抗体特征。

目的:结直肠癌(CRC)已被报道为全球癌症死亡的第二大原因。5年生存率约为50%，主要是由于晚期诊断，早期发现的必要性。本研究旨在检测患者血清中的自身抗体，用于癌症的早期筛查。实验设计:该研究使用高密度人类蛋白质组阵列，其中包含约17,000个重组蛋白。筛查来自健康个体、印度CRC和中东亚洲CRC的血清。进行生物统计学分析以确定显著的自身抗体改变。通过通路分析来探索该疾病的潜在机制。结果:综合蛋白质组学分析显示，CORO7、KCNAB1、WRAP53、NDUFS6、KRT30、COLGALT2等15组蛋白出现异常。进一步的生物通路分析显示，在一些重要的生物通路中，如ECM降解和细胞骨架重塑等都受到了干扰。结论和临床意义:针对癌症相关途径的自身免疫反应的产生可能与疾病的筛查有关。免疫监视的过程可以在癌症的早期阶段检测到。此外，通过最小侵入试验，可以很容易地从血清中提取自身抗体，用于疾病筛查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊