Detection of Apical Dental Resorption Caused by Endodontic Infection in Mice Using Fluorescence and Bright-Field Microscopy.

4区 工程技术 Q3 Physics and Astronomy Scanning Pub Date : 2023-01-01 DOI:10.1155/2023/4619503
Marcio Santos de Carvalho, Luciano Aparecido de Almeida-Junior, Yuri Jivago Silva Ribeiro, Maya Fernanda Manfrin Arnez, Raquel Assed Bezerra da Silva, Fabrício Kitazono de Carvalho, Francisco Wanderley Garcia Paula-Silva
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Abstract

The aim of this study was to evaluate the sensitivity, specificity, and predictive values of the fluorescence microscopy method in the detection of apical dental reabsorption after induction of apical periodontitis in animal models. Forty-first molars of mice, aged 6 to 8 weeks, had their root canals exposed to the oral environment or were maintained healthy as controls (n = 20). After 14 and 42 days, mice were euthanized and tissues were collected for histological evaluation by means of bright field and fluorescence microscopy. The accuracy of fluorescence microscopy in identifying apical external dental resorption was investigated using a diagnostic validation test based on the sensitivity (S) and specificity (E) properties. Bright-field microscopy revealed a higher number of specimens with scores of 1 to 3 - absence of apical dental resorption (n = 29; 52%), while fluorescence microscopy revealed a higher number of specimens with scores of 4 to 6 - presence of apical dental resorption (n = 37; 66%). Out of 56 specimens, 26 were TP, 11 were FP, and 19 were TN. No FN result was observed. Fluorescence microscopy presented a sensitivity value of 1, similar to the bright-field method, while specificity was lower (0.633). The accuracy of the fluorescent method to detect apical dental resorption was 0.804. Fluorescence microscopy revealed a higher number of false positive apical dental resorption than bright-field microscopy. The detection of apical dental resorption was not impacted by the sensitivity of the method but by its specificity.

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荧光和明场显微镜检测小鼠根管感染引起的根尖牙吸收。
本研究的目的是评价荧光显微镜方法在动物模型根尖牙周炎诱导后检测根尖牙重吸收的敏感性、特异性和预测价值。6 ~ 8周龄小鼠的41磨牙将根管暴露于口腔环境或保持健康作为对照组(n = 20)。分别于第14天和第42天对小鼠实施安乐死,取组织进行明光显微镜和荧光显微镜组织学观察。采用基于敏感性(S)和特异性(E)特性的诊断验证试验,研究荧光显微镜鉴别根尖外牙吸收的准确性。明光显微镜显示1到3分的标本数量较多-根尖牙吸收缺失(n = 29;52%),而荧光显微镜显示更多的标本得分为4至6分-存在根尖牙吸收(n = 37;66%)。56例标本中TP 26例,FP 11例,TN 19例,未见FN结果。荧光显微镜的灵敏度为1,与明场法相近,特异度较低(0.633)。荧光法检测根尖牙吸收的准确度为0.804。荧光显微镜显示假阳性的根尖牙吸收比明场显微镜更多。根尖牙吸收的检测不受方法敏感性的影响,而受其特异性的影响。
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来源期刊
Scanning
Scanning 工程技术-显微镜技术
CiteScore
4.40
自引率
0.00%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Scanning provides an international and interdisciplinary medium for the rapid exchange of information among all scientists interested in scanning electron, scanning probe, and scanning optical microscopies. Areas of specific interest include all aspects of the instrumentation associated with scanning microscopies, correlative microscopy techniques, stereometry, stereology, analytic techniques, and novel applications of the microscopies.
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