Identification of Proteins Related to Nickel Homeostasis in Helicobater pylori by Immobilized Metal Affinity Chromatography and Two-Dimensional Gel Electrophoresis.

Xuesong Sun, Ruiguang Ge, Jen-Fu Chiu, Hongzhe Sun, Qing-Yu He
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引用次数: 22

Abstract

Helicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed.

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固定化金属亲和色谱和二维凝胶电泳鉴定幽门螺杆菌中镍稳态相关蛋白。
幽门螺杆菌是一种广泛存在的人类病原体,可引起消化性溃疡和慢性胃炎。维持镍的体内平衡对人类幽门螺杆菌感染的建立至关重要。采用固定化镍亲和层析法从幽门螺杆菌细胞提取物中分离出镍相关蛋白。利用双向凝胶电泳和质谱技术分离鉴定了幽门螺杆菌中22种镍相互作用蛋白。这些与ni相互作用的蛋白可以分为几个一般的功能类别,包括细胞过程(HspA, HspB, TsaA和NapA),酶(脲酶,富马酶,GuaB, Cad, PPase和DmpI),膜相关蛋白(OM jhp1427和HpaA),铁储存蛋白(Pfr)和假设蛋白(HP0271, hpjhp0216, hpjhp0301, HP0721, HP0614和hpjhp0118)。讨论了这些蛋白在镍稳态中的意义。
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